Evidence map›Paper›PMID 27344519›Full record

ReviewCurrent opinion in biotechnology2016

Transcriptional regulation with CRISPR-Cas9: principles, advances, and applications.

Andriy Didovyk, Bartłomiej Borek, Lev Tsimring, Jeff Hasty

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Current opinion in biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Analysis of Transcriptional Regulation in Bone Cells.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  7. Article
  8. Review
  9. Article
  10. Review
  11. Review
  12. Knockout mutation inFrontiers in genome editing · 2024
    Article
  13. Review
  14. Review
  15. Article
  16. Review
  17. Article
  18. Tips, Tricks, and Potential Pitfalls of CRISPR Genome Editing inFrontiers in bioengineering and biotechnology · 2022
    Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Andriy DidovykBioCircuits Institute, University of California San Diego, La Jolla, CA 92093, USA; San Diego Center for Systems Biology, University of California San Diego, La Jolla, CA 92093, USA.
Bartłomiej BorekBioCircuits Institute, University of California San Diego, La Jolla, CA 92093, USA; San Diego Center for Systems Biology, University of California San Diego, La Jolla, CA 92093, USA.
Lev TsimringBioCircuits Institute, University of California San Diego, La Jolla, CA 92093, USA; San Diego Center for Systems Biology, University of California San Diego, La Jolla, CA 92093, USA. Electronic address: ltsimring@ucsd.edu.
Jeff HastyBioCircuits Institute, University of California San Diego, La Jolla, CA 92093, USA; San Diego Center for Systems Biology, University of California San Diego, La Jolla, CA 92093, USA; Department of Bioengineering, University of California San Diego, La Jolla, CA 92093, USA; Molecular Biology Section, Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA. Electronic address: jhasty@eng.ucsd.edu.

Funding

SYNTHETIC SYSTEMSP50GM085764 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HASTY, JEFF M, IDEKER, TREY · 2010 to 2018
$23.3M
Engineered Gene Circuits for Basic Science and BiotechnologyR01GM069811 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HASTY, JEFF M, TSIMRING, LEV S · 2004 to 2025
$13.6M
NIGMS NIH HHS P50 GM085764NIGMS NIH HHS R01 GM069811
6 · The paper itself

Abstract

CRISPR-Cas9 has recently emerged as a promising system for multiplexed genome editing as well as epigenome and transcriptome perturbation. Due to its specificity, ease of use and highly modular programmable nature, it has been widely adopted for a variety of applications such as genome editing, transcriptional inhibition and activation, genetic screening, DNA localization imaging, and many more. In this review, we will discuss non-editing applications of CRISPR-Cas9 for transcriptome perturbation, metabolic engineering, and synthetic biology.

Indexed as

Gene EditingGene Expression RegulationTranscription, GeneticAnimalsCRISPR-Cas SystemsHumans

Identifiers

PMID27344519
PMCPMC4975649

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.