ArticleCytotechnology2016
LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds.
Article in Cytotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
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- Study on antihepatocellular carcinoma effect of 6-shogaol and curcumin through network-based pharmacological and cellular assay.Frontiers in pharmacology · 2024Article
- Assessment of anticancer properties of cumin seed (Frontiers in oncology · 2023Article
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- Aglycone flavonoid brachydin A shows selective cytotoxicity and antitumoral activity in human metastatic prostate (DU145) cancer cells.Cytotechnology · 2021Article
- Carvacrol Promotes Cell Cycle Arrest and Apoptosis through PI3K/AKT Signaling Pathway in MCF-7 Breast Cancer Cells.Chinese journal of integrative medicine · 2021Article
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- Composite Bioinks With Mesoporous Bioactive Glasses-A Critical Evaluation of Results Obtained byFrontiers in bioengineering and biotechnology · 2021Article
- Dose Dependent Antimicrobial Cellular Cytotoxicity-Implications forFrontiers in pharmacology · 2021Article
- α-Pinene Induces Apoptotic Cell Death via Caspase Activation in Human Ovarian Cancer Cells.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- A Comparative Evaluation of the Cytotoxic and Antioxidant Activity ofOxidative medicine and cellular longevity · 2018Article
- Cytotoxicity of Propolis Extracts obtained using Dichloromethane and Hexane Solvent on Human Salivary Gland Tumor Cell Line.Journal of International Society of Preventive & Community DentistryArticle
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brazilian flora biodiversity has been widely investigated to identify effective and safe phytotherapeutic compounds. Among the investigated plant species, the Byrsonima genus exhibits promising biological activities. This study aimed at evaluating the cytotoxicity of B. correifolia, B. verbascifolia, B. fagifolia and B. intermedia extracts using different assays in two cell lines (primary gastric and HepG2 cells). The different extract concentrations effects on cell viability were assayed using the MTT, aquabluer, neutral red and LDH assays. Non-cytotoxic concentrations were selected to generate cell proliferation curves and to assess cell cycle kinetics by flow cytometry. Byrsonima extracts differentially affected cell viability depending on the metabolic cellular state and the biological parameter evaluated. B. fagifolia and B. intermedia extracts exhibited lower cytotoxic effects than B. correifolia and B. verbascifolia in all assays. The results obtained with LDH and flow cytometry assays were more reliable, suggesting that they can be useful in the screening for herbal medicine and to further characterize these extracts as phytotherapeutic compounds.
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Registered trials
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