ArticleBMC microbiology2016
Identification of the crp gene in avian Pasteurella multocida and evaluation of the effects of crp deletion on its phenotype, virulence and immunogenicity.
Article in BMC microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 59 citations in OpenAlex.
- Comparative analysis of shared and unique mechanisms important for diverse strains of Pasteurella multocida to cause systemic infection in mice.PLoS pathogens · 2025Article
- Attenuated vaccine PmCQ2Δ4555-4580 effectively protects mice against Pasteurella multocida infection.BMC veterinary research · 2024Article
- The cAMP Receptor Protein (CRP) ofAnimals : an open access journal from MDPI · 2024Article
- Global commercialization and research of veterinary vaccines againstVeterinary world · 2023Article
- Immunogenicity and protection of a Pasteurella multocida strain with a truncated lipopolysaccharide outer core in ducks.Veterinary research · 2022Article
- The lipopolysaccharide outer core transferase genes pcgD and hptE contribute differently to the virulence of Pasteurella multocida in ducks.Veterinary research · 2021Article
- Effect of cAMP Receptor Protein Gene on Growth Characteristics and Stress Resistance ofFrontiers in cellular and infection microbiology · 2020Article
- l-Serine Lowers the Inflammatory Responses during Pasteurella multocida Infection.Infection and immunity · 2019Article
- Isolate Specific Cold Response ofFrontiers in microbiology · 2019Article
- Identification of Fur inFrontiers in veterinary science · 2019Article
- cAMP Receptor Protein of Salmonella enterica Serovar Typhimurium Modulate Glycolysis in Macrophages to Induce Cell Apoptosis.Current microbiology · 2019Article
- The Enhancement of the Pasteurella's Bacterin by Propolis Extracts.Reports of biochemistry & molecular biology · 2018Article
- Roles of theCanadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2018Article
- Therapeutic Application of Bacteriophage PHB02 and Its Putative Depolymerase AgainstFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPasteurella multocida (P. multocida) is an important veterinary pathogen that can cause severe diseases in a wide range of mammals and birds. The global regulator crp gene has been found to regulate the virulence of some bacteria, and crp mutants have been demonstrated to be effective attenuated vaccines against Salmonella enterica and Yersinia enterocolitica. Here, we first characterized the crp gene in P. multocida, and we report the effects of a crp deletion.
resultsThe P. multocida crp mutant exhibited a similar lipopolysaccharide and outer membrane protein profile but displayed defective growth and serum complement resistance in vitro compared with the parent strain. Furthermore, crp deletion decreased virulence but did not result in full attenuation. The 50 % lethal dose (LD50) of the Δcrp mutant was 85-fold higher than that of the parent strain for intranasal infection. Transcriptome sequencing analysis showed that 92 genes were up-regulated and 94 genes were down-regulated in the absence of the crp gene. Finally, we found that intranasal immunization with the Δcrp mutant triggered both systematic and mucosal antibody responses and conferred 60 % protection against virulent P. multocida challenge in ducks.
conclusionThe deletion of the crp gene has an inhibitory effect on bacterial growth and bacterial resistance to serum complement in vitro. The P. multocida crp mutant was attenuated and conferred moderate protection in ducks. This work affords a platform for analyzing the function of crp and aiding the formulation of a novel vaccine against P. multocida.
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