ArticleAmerican journal of physiology. Heart and circulatory physiology2016
Zebrafish heart as a model to study the integrative autonomic control of pacemaker function.
Article in American journal of physiology. Heart and circulatory physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 57 citations in OpenAlex.
- Heart rate control by listening to light.Nature communications · 2026Article
- Distinct transcriptional programs define cranial motor neuron subtypes during vertebrate development.bioRxiv : the preprint server for biology · 2026Article
- Acute severe hypoxia induces prolonged cardiac transcriptomic remodeling despite rapid functional recovery.Research square · 2026Article
- Zebrafish as a Model for Cardiovascular Disease Using Nanotechnology and Emerging Optogenetic Tools.Biomedicines · 2026Review
- Synchronization of behavioral and cardiac dynamics in larval zebrafish.Cell reports · 2026Article
- Sublethal cadmium exposure disrupts cardiac function and tissue structure in zebrafish larvae.Frontiers in physiology · 2026Article
- Cholinergic suppression of heart rate acceleration during intrinsic optogenetic activation of sympathetic cardiac neurons in perfused hearts.American journal of physiology. Heart and circulatory physiology · 2025Article
- Emergence of Functional Heart-Brain Circuits in a Vertebrate.bioRxiv : the preprint server for biology · 2025Article
- Regulation of Cardiac Function by the Autonomic Nervous System.Physiology (Bethesda, Md.) · 2025Review
- Decoding the molecular, cellular, and functional heterogeneity of zebrafish intracardiac nervous system.Nature communications · 2024Article
- scRNA-seq reveals the diversity of the developing cardiac cell lineage and molecular players in heart rhythm regulation.iScience · 2024Article
- Article
- Zebrafish as a high throughput model for organ preservation and transplantation research.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- TheFrontiers in physiology · 2023Article
- Regulation of heart rate following genetic deletion of the ß1 adrenergic receptor in larval zebrafish.Acta physiologica (Oxford, England) · 2022Article
- The Effect of Hypothermia and Osmotic Shock on the Electrocardiogram of Adult Zebrafish.Biology · 2022Article
- KHz-rate volumetric voltage imaging of the whole Zebrafish heart.Biophysical reports · 2022Article
- Drivers of Sinoatrial Node Automaticity in Zebrafish: Comparison With Mechanisms of Mammalian Pacemaker Function.Frontiers in physiology · 2022Article
- The effective use of blebbistatin to study the action potential of cardiac pacemaker cells of zebrafish (Current research in physiology · 2022Article
- From Mice to Mainframes: Experimental Models for Investigation of the Intracardiac Nervous System.Journal of cardiovascular development and disease · 2021Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cardiac pacemaker sets the heart's primary rate, with pacemaker discharge controlled by the autonomic nervous system through intracardiac ganglia. A fundamental issue in understanding the relationship between neural activity and cardiac chronotropy is the identification of neuronal populations that control pacemaker cells. To date, most studies of neurocardiac control have been done in mammalian species, where neurons are embedded in and distributed throughout the heart, so they are largely inaccessible for whole-organ, integrative studies. Here, we establish the isolated, innervated zebrafish heart as a novel alternative model for studies of autonomic control of heart rate. Stimulation of individual cardiac vagosympathetic nerve trunks evoked bradycardia (parasympathetic activation) and tachycardia (sympathetic activation). Simultaneous stimulation of both vagosympathetic nerve trunks evoked a summative effect. Effects of nerve stimulation were mimicked by direct application of cholinergic and adrenergic agents. Optical mapping of electrical activity confirmed the sinoatrial region as the site of origin of normal pacemaker activity and identified a secondary pacemaker in the atrioventricular region. Strong vagosympathetic nerve stimulation resulted in a shift in the origin of initial excitation from the sinoatrial pacemaker to the atrioventricular pacemaker. Putative pacemaker cells in the sinoatrial and atrioventricular regions expressed adrenergic β2 and cholinergic muscarinic type 2 receptors. Collectively, we have demonstrated that the zebrafish heart contains the accepted hallmarks of vertebrate cardiac control, establishing this preparation as a viable model for studies of integrative physiological control of cardiac function by intracardiac neurons.
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