ArticleBMC veterinary research2016
Antimicrobial synergy between carprofen and doxycycline against methicillin-resistant Staphylococcus pseudintermedius ST71.
Article in BMC veterinary research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Synthesis Methods and Therapeutic Journey of Carprofen and Its Derivatives: A Review.Chemical biology & drug design · 2025Review
- Clinical significance of microbiota changes under the influence of psychotropic drugs. An updated narrative review.Frontiers in microbiology · 2023Review
- Clostridioides difficile Modifies its Aromatic Compound Metabolism in Response to Amidochelocardin-Induced Membrane Stress.mSphere · 2022Article
- In Silico and Experimental Investigation of the Biological Potential of Some Recently Developed Carprofen Derivatives.Molecules (Basel, Switzerland) · 2022Article
- Evaluation of the effects of chlorhexidine digluconate with and without cBD103 or cCath against multidrug-resistant clinical isolates of Staphylococcus pseudintermedius.Veterinary dermatology · 2022Article
- From canines to humans: Clinical importance of Staphylococcus pseudintermedius.PLoS pathogens · 2021Article
- Synergistic Effects of Thiosemicarbazides with Clinical Drugs againstMolecules (Basel, Switzerland) · 2020Article
- Comparison of methods for the detection of in vitro synergy in multidrug-resistant gram-negative bacteria.BMC microbiology · 2020Article
- Synergistic ROS-Associated Antimicrobial Activity of Silver Nanoparticles and Gentamicin AgainstInternational journal of nanomedicine · 2020Article
- Re-sensitizing Ampicillin and Kanamycin-ResistantFrontiers in bioengineering and biotechnology · 2020Article
- Carprofen-induced depletion of proton motive force reverses TetK-mediated doxycycline resistance in methicillin-resistant Staphylococcus pseudintermedius.Scientific reports · 2019Article
- Structure⁻Activity Study, Characterization, and Mechanism of Action of an Antimicrobial Peptoid D2 and Its d- and l-Peptide Analogues.Molecules (Basel, Switzerland) · 2019Article
- Characterization, mechanism of action and optimization of activity of a novel peptide-peptoid hybrid against bacterial pathogens involved in canine skin infections.Scientific reports · 2019Article
- Drug combinations: a strategy to extend the life of antibiotics in the 21st century.Nature reviews. Microbiology · 2019Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNew therapeutic strategies are needed to face the rapid spread of multidrug-resistant staphylococci in veterinary medicine. The objective of this study was to identify synergies between antimicrobial and non-antimicrobial drugs commonly used in companion animals as a possible strategy to restore antimicrobial susceptibility in methicillin-resistant Staphylococcus pseudintermedius (MRSP).
resultsA total of 216 antimicrobial/non-antimicrobial drug combinations were screened by disk diffusion using a clinical MRSP sequence type (ST) 71 strain resistant to all six antimicrobials tested (ampicillin, ciprofloxacin, clindamycin, doxycycline, oxacillin and trimethoprim/sulfamethoxazole). The most promising drug combination (doxycycline-carprofen) was further assessed by checkerboard testing extended to four additional MRSP strains belonging to ST71 or ST68, and by growth inhibition experiments. Seven non-antimicrobial drugs (bromhexine, acepromazine, amitriptyline, clomipramine, carprofen, fluoxetine and ketoconazole) displayed minimum inhibitory concentrations (MICs) ranging between 32 and >4096 mg/L, and enhanced antimicrobial activity of one or more antimicrobials. Secondary screening by checkerboard assay revealed a synergistic antimicrobial effect between carprofen and doxycycline, with the sum of the fractional inhibitory concentration indexes (ΣFICI) ranging between 0.3 and 0.5 depending on drug concentration. Checkerboard testing of multiple MRSP strains revealed a clear association between synergy and carriage of tetK, which is a typical feature of MRSP ST71. An increased growth inhibition was observed when MRSP ST71 cells in exponential phase were exposed to 0.5/32 mg/L of doxycycline/carprofen compared to individual drug exposure.
conclusionsCarprofen restores in vitro susceptibility to doxycycline in S. pseudintermedius strains carrying tetK such as MRSP ST71. Further research is warranted to elucidate the molecular mechanism behind the identified synergy and its linkage to tetK.
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