ArticleJournal of visualized experiments : JoVE2016
Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 25 citations in OpenAlex.
- Targeting CSF-1 signaling between tumor cells and macrophages at TMEM doorways inhibits breast cancer dissemination.Oncogene · 2025Article
- Protective effects of liraglutide on hypercholesterolemia-associated atherosclerosis involve attenuation of endothelial-monocyte adhesion through down-regulating the LOX-1/NF-κB signaling pathway.Scientific reports · 2025Article
- Article
- Signaling events at TMEM doorways provide potential targets for inhibiting breast cancer dissemination.bioRxiv : the preprint server for biology · 2024Article
- Stabilized Window for Intravital Imaging of the Murine Pancreas.Journal of visualized experiments : JoVE · 2023Article
- Multidimensional Imaging of Breast Cancer.Cold Spring Harbor perspectives in medicine · 2023Review
- Assessment of MRI to estimate metastatic dissemination risk and prometastatic effects of chemotherapy.NPJ breast cancer · 2022Article
- SWIP-a stabilized window for intravital imaging of the murine pancreas.Open biology · 2022Article
- A Permanent Window for Investigating Cancer Metastasis to the Lung.Journal of visualized experiments : JoVE · 2021Article
- Intravital microscopy of dynamic single-cell behavior in mouse mammary tissue.Nature protocols · 2021Article
- The Cellular Organization of the Mammary Gland: Insights From Microscopy.Journal of mammary gland biology and neoplasia · 2021Review
- The role of the tumor microenvironment in tumor cell intravasation and dissemination.European journal of cell biology · 2020Article
- Validation of an Automated Quantitative Digital Pathology Approach for Scoring TMEM, a Prognostic Biomarker for Metastasis.Cancers · 2020Article
- Hematogenous Dissemination of Breast Cancer Cells From Lymph Nodes Is Mediated by Tumor MicroEnvironment of Metastasis Doorways.Frontiers in oncology · 2020Article
- Invadopodia-mediated ECM degradation is enhanced in the G1 phase of the cell cycle.Journal of cell science · 2019Article
- The emerging roles of macrophages in cancer metastasis and response to chemotherapy.Journal of leukocyte biology · 2019Review
- Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis.Journal of visualized experiments : JoVE · 2019Article
- Chemotherapy-induced metastasis: mechanisms and translational opportunities.Clinical & experimental metastasis · 2018Review
- Emerging roles for LPP in metastatic cancer progression.Journal of cell communication and signaling · 2018Review
- Time-lapsed, large-volume, high-resolution intravital imaging for tissue-wide analysis of single cell dynamics.Methods (San Diego, Calif.) · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In the tumor microenvironment, host stromal cells interact with tumor cells to promote tumor progression, angiogenesis, tumor cell dissemination and metastasis. Multicellular interactions in the tumor microenvironment can lead to transient events including directional tumor cell motility and vascular permeability. Quantification of tumor vascular permeability has frequently used end-point experiments to measure extravasation of vascular dyes. However, due to the transient nature of multicellular interactions and vascular permeability, the kinetics of these dynamic events cannot be discerned. By labeling cells and vasculature with injectable dyes or fluorescent proteins, high-resolution time-lapse intravital microscopy has allowed the direct, real-time visualization of transient events in the tumor microenvironment. Here we describe a method for using multiphoton microscopy to perform extended intravital imaging in live mice to directly visualize multicellular dynamics in the tumor microenvironment. This method details cellular labeling strategies, the surgical preparation of a mammary skin flap, the administration of injectable dyes or proteins by tail vein catheter and the acquisition of time-lapse images. The time-lapse sequences obtained from this method facilitate the visualization and quantitation of the kinetics of cellular events of motility and vascular permeability in the tumor microenvironment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.