ArticleJournal of visualized experiments : JoVE2016
A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
13 citing papers in PubMed.
- Usurpativirus massiliensis, a new giant virus related to clandestinovirus.Virology journal · 2025Article
- Asfarviruses and Closely Related Giant Viruses.Viruses · 2023Review
- Genome Sequences of Two New Pandoravirus Strains Isolated from Brazil and France.Microbiology resource announcements · 2022Article
- Diversity of Giant Viruses InfectingFrontiers in microbiology · 2022Review
- Article
- Clandestinovirus: A Giant Virus With Chromatin Proteins and a Potential to Manipulate the Cell Cycle of Its HostFrontiers in microbiology · 2021Article
- Article
- Review
- High-throughput isolation of giant viruses using high-content screening.Communications biology · 2019Article
- Pacmanvirus, a New Giant Icosahedral Virus at the Crossroads between Asfarviridae and Faustoviruses.Journal of virology · 2017Article
- Genome Sequences of New Faustovirus Strains ST1 and LC9, Isolated from the South of France.Genome announcements · 2017Article
- Orpheovirus IHUMI-LCC2: A New Virus among the Giant Viruses.Frontiers in microbiology · 2017Article
- Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses.Viruses · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The isolation of giant viruses is of great interest in this new era of virology, especially since these giant viruses are related to protists. Giant viruses may be potentially pathogenic for many species of protists. They belong to the recently described order of Megavirales. The new lineage Faustovirus that has been isolated from sewage samples is distantly related to the mammalian pathogen African swine fever virus. This virus is also specific to its amoebal host, Vermamoeba vermiformis, a protist common in health care water systems. It is crucial to continue isolating new Faustovirus genotypes in order to enlarge its genotype collection and study its pan-genome. We developed new strategies for the isolation of additional strains by improving the use of antibiotic and antifungal combinations in order to avoid bacterial and fungal contaminations of the amoeba co-culture and favoring the virus multiplication. We also implemented a new starvation medium to maintain V. vermiformis in optimal conditions for viruses co-culture. Finally, we used flow cytometry rather than microscopic observation, which is time-consuming, to detect the cytopathogenic effect. We obtained two isolates from sewage samples, proving the efficiency of this method and thus widening the collection of Faustoviruses, to better understand their environment, host specificity and genetic content.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.