ArticlePloS one2016
Molecular Dynamics Driven Design of pH-Stabilized Mutants of MNEI, a Sweet Protein.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Expression of a single-chain monellin (MNEI) mutant with enhanced stability in transgenic mice milk.Transgenic research · 2024Article
- Computational design of a cutinase for plastic biodegradation by mining molecular dynamics simulations trajectories.Computational and structural biotechnology journal · 2022Article
- A Super Stable Mutant of the Plant Protein Monellin Endowed with Enhanced Sweetness.Life (Basel, Switzerland) · 2021Article
- Metabolic Effects of the Sweet Protein MNEI as a Sweetener in Drinking Water. A Pilot Study of a High Fat Dietary Regimen in a Rodent Model.Nutrients · 2019Article
- Effects of Sweeteners on the Gut Microbiota: A Review of Experimental Studies and Clinical Trials.Advances in nutrition (Bethesda, Md.) · 2019Article
- Structural effects of methylglyoxal glycation, a study on the model protein MNEI.Molecular and cellular biochemistry · 2019Article
- Getting value from the waste: recombinant production of a sweet protein by Lactococcus lactis grown on cheese whey.Microbial cell factories · 2018Article
- High-level production of single chain monellin mutants with enhanced sweetness and stability in tobacco chloroplasts.Planta · 2018Article
- Molecular dynamics-derived rotamer libraries for d-amino acids within homochiral and heterochiral polypeptides.Protein engineering, design & selection : PEDS · 2018Article
- Enhanced Thermostability of Glucose Oxidase through Computer-Aided Molecular Design.International journal of molecular sciences · 2018Article
- Insights from molecular dynamics simulations for computational protein design.Molecular systems design & engineering · 2017Article
- Sweeter and stronger: enhancing sweetness and stability of the single chain monellin MNEI through molecular design.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MNEI is a single chain derivative of monellin, a plant protein that can interact with the human sweet taste receptor, being therefore perceived as sweet. This unusual physiological activity makes MNEI a potential template for the design of new sugar replacers for the food and beverage industry. Unfortunately, applications of MNEI have been so far limited by its intrinsic sensitivity to some pH and temperature conditions, which could occur in industrial processes. Changes in physical parameters can, in fact, lead to irreversible protein denaturation, as well as aggregation and precipitation. It has been previously shown that the correlation between pH and stability in MNEI derives from the presence of a single glutamic residue in a hydrophobic pocket of the protein. We have used molecular dynamics to study the consequences, at the atomic level, of the protonation state of such residue and have identified the network of intramolecular interactions responsible for MNEI stability at acidic pH. Based on this information, we have designed a pH-independent, stabilized mutant of MNEI and confirmed its increased stability by both molecular modeling and experimental techniques.
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