Evidence map›Paper›PMID 27340655›Full record

ArticleBioMed research international2016

Phosphoprotein Phosphatase 1 Is Required for Extracellular Calcium-Induced Keratinocyte Differentiation.

Chandrama Shrestha, Yuanyuan Tang, Hong Fan, Lusha Li, Qin Zeng, Sally D Pennypacker, Daniel D Bikle, Zhongjian Xie

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioMed research international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.4field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Chandrama ShresthaInstitute of Endocrinology and Metabolism, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.ORCID 0000-0002-5653-9887
Yuanyuan TangInstitute of Endocrinology and Metabolism, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.ORCID 0000-0003-3956-5685
Hong FanInstitute of Endocrinology and Metabolism, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
Lusha LiInstitute of Endocrinology and Metabolism, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.ORCID 0000-0003-1863-8603
Qin ZengInstitute of Endocrinology and Metabolism, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
Sally D PennypackerEndocrine Unit, Veterans Affairs Medical Center, University of California, San Francisco, CA 94121, USA.
Daniel D BikleEndocrine Unit, Veterans Affairs Medical Center, University of California, San Francisco, CA 94121, USA.ORCID 0000-0002-1040-475X
Zhongjian XieInstitute of Endocrinology and Metabolism, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China; Endocrine Unit, Veterans Affairs Medical Center, University of California, San Francisco, CA 94121, USA.
Central South University · CNSan Francisco VA Medical Center · US

Funding

Vitamin D Receptor Coactivators in KeratinocytesR01AR050023 · NIAMS · NORTHERN CALIFORNIA INSTITUTE RES &EDUC · PI BIKLE, DANIEL DAVID · 2004 to 2014
$3.4M
VITAMIN D AND THE DIFFERENTIATION OF KERATINOCYTESR01AR038386 · NIAMS · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI BIKLE, DANIEL DAVID · 1987 to 2003
$1.1M
Role of p120-catenin in oral squamous cell carcinomaR21DE019529 · NIDCR · NORTHERN CALIFORNIA INSTITUTE/RES/EDU · PI XIE, ZHONGJIAN · 2009 to 2010
$424k
The role of PIKE-S in mediating EGFR mitogenic signaling in OSCC cellsR03DE018001 · NIDCR · NORTHERN CALIFORNIA INSTITUTE/RES/EDU · PI XIE, ZHONGJIAN · 2007 to 2008
$157k
Role of vitamin D and calcium signaling in wound healingI01BX001066 · VA · VETERANS AFFAIRS MED CTR SAN FRANCISCO · PI BIKLE, DANIEL DAVID · 2011 to 2014
–
BLRD VA I01 BX001066NIAMS NIH HHS R01 AR038386NIAMS NIH HHS R01 AR050023NIDCR NIH HHS R03 DE018001NIDCR NIH HHS R21 DE019529
6 · The paper itself

Abstract

Extracellular calcium is a major regulator of keratinocyte differentiation in vitro and appears to play that role in vivo, but the mechanism is unclear. We have previously demonstrated that, following calcium stimulation, PIP5K1α is recruited by the E-cadherin-β-catenin complex to the plasma membrane where it provides the substrate PIP2 for both PI3K and PLC-γ1. This signaling pathway is critical for calcium-induced generation of second messengers including IP3 and intracellular calcium and keratinocyte differentiation. In this study, we explored the upstream regulatory mechanism by which calcium activates PIP5K1α and the role of this activation in calcium-induced keratinocyte differentiation. We found that treatment of human keratinocytes in culture with calcium resulted in an increase in serine dephosphorylation and PIP5K1α activation. PP1 knockdown blocked extracellular calcium-induced increase in serine dephosphorylation and activity of PIP5K1α and induction of keratinocyte differentiation markers. Knockdown of PLC-γ1, the downstream effector of PIP5K1α, blocked upstream dephosphorylation and PIP5K1α activation induced by calcium. Coimmunoprecipitation revealed calcium induced recruitment of PP1 to the E-cadherin-catenin-PIP5K1α complex in the plasma membrane. These results indicate that PP1 is recruited to the extracellular calcium-dependent E-cadherin-catenin-PIP5K1α complex in the plasma membrane to activate PIP5K1α, which is required for PLC-γ1 activation leading to keratinocyte differentiation.

Indexed as

beta CateninCadherinsCalciumCell DifferentiationCells, CulturedGene Expression Regulation, DevelopmentalGene Knockdown TechniquesHumansKeratinocytesPhospholipase C gammaPhosphotransferases (Alcohol Group Acceptor)Protein Phosphatase 1Receptors, Neuropeptide YSignal Transduction1-phosphatidylinositol-4-phosphate 5-kinasebeta CateninCadherinsCalciumneuropeptide Y4 receptorPhospholipase C gammaPhosphotransferases (Alcohol Group Acceptor)Protein Phosphatase 1Receptors, Neuropeptide Y

Identifiers

PMID27340655
PMCPMC4909930
OpenAlexW2415474540

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.