ArticleBioImpacts : BI2016
Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture.
Article in BioImpacts : BI, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Role of Collection Media on the Biological Activity of Extracellular Vesicles From hTERT-Immortalised Mesenchymal Stromal Cells.Journal of extracellular vesicles · 2026Article
- Deep autoencoder as an interpretable tool for Raman spectroscopy investigation of chemical and extracellular vesicle mixtures.Biomedical optics express · 2024Article
- Cell culture-derived extracellular vesicles: Considerations for reporting cell culturing parameters.Journal of extracellular biology · 2023Article
- Exosomes induce neurogenesis of pluripotent P19 cells.Stem cell reviews and reports · 2023Article
- Chondrogenic Potential of Human Umbilical Cord Mesenchymal Stem Cells Cultured with Exosome-Depleted Fetal Bovine Serum in an Osteoarthritis Mouse Model.Biomedicines · 2022Article
- The dark side of foetal bovine serum in extracellular vesicle studies.Journal of extracellular vesicles · 2022Review
- Magnetofluoro-Immunosensing Platform Based on Binary Nanoparticle-Decorated Graphene for Detection of Cancer Cell-Derived Exosomes.International journal of molecular sciences · 2022Article
- Considerations for extracellular vesicle and lipoprotein interactions in cell culture assays.Journal of extracellular vesicles · 2022Article
- Isolation, characterization, and functional study of extracellular vesicles derived fromFrontiers in cellular and infection microbiology · 2022Article
- Electrophysiological engineering of heart-derived cells with calcium-dependent potassium channels improves cell therapy efficacy for cardioprotection.Nature communications · 2021Article
- Assessing extracellular vesicles from bovine mammary gland epithelial cells cultured in FBS-free medium.Extracellular vesicles and circulating nucleic acids · 2021Article
- Foetal bovine serum influence on in vitro extracellular vesicle analyses.Journal of extracellular vesicles · 2021Article
- Tumor-derived extracellular vesicles: insights into bystander effects of exosomes after irradiation.Lasers in medical science · 2020Review
- Bone Metastasis Phenotype and Growth Undergo Regulation by Micro-Environment Stimuli: Efficacy of Early Therapy with HGF or TGFβ1-Type I Receptor Blockade.International journal of molecular sciences · 2019Article
- Response to "Technical approaches to reduce interference of Fetal calf serum derived RNA in the analysis of extracellular vesicle RNA from cultured cells".Journal of extracellular vesicles · 2019Article
- Roles and Regulation of Extracellular Vesicles in Cardiovascular Mineral Metabolism.Frontiers in cardiovascular medicine · 2018Review
- Efficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serum.Journal of extracellular vesicles · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionCardiac progenitor cells (CPCs) represent a powerful tool in cardiac regenerative medicine. Pre-clinical studies suggest that most of the beneficial effects promoted by the injected cells are due to their paracrine activity exerted on endogenous cells and tissue. Exosomes are candidate mediators of this paracrine effects. According to their potential, many researchers have focused on characterizing exosomes derived from specific cell types, but, up until now, only few studies have analyzed the possible in vitro effects of bovine serum-derived exosomes on cell proliferation or differentiation.
methodsThe aim of this study was to analyse, from a qualitative and quantitative point of view, the in vitro effects of bovine serum exosomes on human CPCs cultured either as cardiospheres or as monolayers of cardiosphere-forming cells.
resultsEffects on proliferation, yield and molecular patterning were detected. We show, for the first time, that exogenous bovine exosomes support the proliferation and migration of human cardiosphere-forming cells, and that their depletion affects cardiospheres formation, in terms of size, yield and extra-cellular matrix production.
conclusionThese results stress the importance of considering differential biological effects of exogenous cell culture supplements on the final phenotype of primary human cell cultures.
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