Evidence map›Paper›PMID 27340568›Full record

ArticleGenetics research international2016

Assessment of Functional EST-SSR Markers (Sugarcane) in Cross-Species Transferability, Genetic Diversity among Poaceae Plants, and Bulk Segregation Analysis.

Shamshad Ul Haq, Pradeep Kumar, R K Singh, Kumar Sambhav Verma, Ritika Bhatt, Meenakshi Sharma, Sumita Kachhwaha, S L Kothari

Open access · hybridAbstract read
In one paragraph

Article in Genetics research international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
7.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 26 citations in OpenAlex.

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  7. Development of EST-SSR markers forPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Shamshad Ul HaqBiotechnology Division, UP Council of Sugarcane Research, Shahjahanpur 242001, India; Interdisciplinary Programme of Life Science for Advance Research and Education, University of Rajasthan, Jaipur 302004, India; Department of Botany, University of Rajasthan, Jaipur 302015, India.
Pradeep KumarBiotechnology Division, UP Council of Sugarcane Research, Shahjahanpur 242001, India; School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
R K SinghBiotechnology Division, UP Council of Sugarcane Research, Shahjahanpur 242001, India.
Kumar Sambhav VermaAmity Institute of Biotechnology, Amity University Rajasthan, Jaipur 302006, India.
Ritika BhattInterdisciplinary Programme of Life Science for Advance Research and Education, University of Rajasthan, Jaipur 302004, India; Department of Botany, University of Rajasthan, Jaipur 302015, India.
Meenakshi SharmaDepartment of Botany, University of Rajasthan, Jaipur 302015, India.
Sumita KachhwahaDepartment of Botany, University of Rajasthan, Jaipur 302015, India.
S L KothariInterdisciplinary Programme of Life Science for Advance Research and Education, University of Rajasthan, Jaipur 302004, India; Department of Botany, University of Rajasthan, Jaipur 302015, India; Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur 302006, India.
University of Rajasthan · INUttar Pradesh Council of sugarcane research · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Expressed sequence tags (ESTs) are important resource for gene discovery, gene expression and its regulation, molecular marker development, and comparative genomics. We procured 10000 ESTs and analyzed 267 EST-SSRs markers through computational approach. The average density was one SSR/10.45 kb or 6.4% frequency, wherein trinucleotide repeats (66.74%) were the most abundant followed by di- (26.10%), tetra- (4.67%), penta- (1.5%), and hexanucleotide (1.2%) repeats. Functional annotations were done and after-effect newly developed 63 EST-SSRs were used for cross transferability, genetic diversity, and bulk segregation analysis (BSA). Out of 63 EST-SSRs, 42 markers were identified owing to their expansion genetics across 20 different plants which amplified 519 alleles at 180 loci with an average of 2.88 alleles/locus and the polymorphic information content (PIC) ranged from 0.51 to 0.93 with an average of 0.83. The cross transferability ranged from 25% for wheat to 97.22% for Schlerostachya, with an average of 55.86%, and genetic relationships were established based on diversification among them. Moreover, 10 EST-SSRs were recognized as important markers between bulks of pooled DNA of sugarcane cultivars through BSA. This study highlights the employability of the markers in transferability, genetic diversity in grass species, and distinguished sugarcane bulks.

Identifiers

PMID27340568
PMCPMC4908241
OpenAlexW2417257983

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.