ArticleThe Journal of endocrinology2016
Daily leptin blunts marrow fat but does not impact bone mass in calorie-restricted mice.
Article in The Journal of endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 58 citations in OpenAlex.
- Trial
- Weight loss induced bone loss: mechanism of action and clinical implications.Bone research · 2025Review
- Bone Marrow Adipocytes as Novel Regulators of Metabolic Homeostasis: Clinical Consequences of Bone Marrow Adiposity.Current obesity reports · 2025Review
- Article
- Nutrition and Bone Marrow Adiposity in Relation to Bone Health.Physiological research · 2024Review
- Calorie restriction in mice impairs cortical but not trabecular peak bone mass by suppressing bone remodeling.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2024Article
- Deletion of Hsd11b1 suppresses caloric restriction-induced bone marrow adiposity in male but not female mice.The Journal of endocrinology · 2024Article
- The Effects of Different Dietary Patterns on Bone Health.Nutrients · 2024Review
- Deficiency of glucocorticoid receptor in bone marrow adipocytes has mild effects on bone and hematopoiesis but does not influence expansion of marrow adiposity with caloric restriction.Frontiers in endocrinology · 2024Article
- A cross-sectional study from NHANES found a positive association between obesity with bone mineral density among postmenopausal women.BMC endocrine disorders · 2023Article
- Turning the spotlight on bone marrow adipocytes in haematological malignancy and non-malignant conditions.British journal of haematology · 2023Review
- New Insights into Calorie Restriction Induced Bone Loss.Endocrinology and metabolism (Seoul, Korea) · 2023Article
- The Role of Bone Cell Energetics in Altering Bone Quality and Strength in Health and Disease.Current osteoporosis reports · 2023Review
- The effects of popular diets on bone health in the past decade: a narrative review.Frontiers in endocrinology · 2023Review
- Bone marrow adipose tissue in metabolic health.Trends in endocrinology and metabolism: TEM · 2022Review
- Effect of obesity status on adolescent bone mineral density and saturation effect: A cross-sectional study.Frontiers in endocrinology · 2022Article
- Ultra-Processed Food Impairs Bone Quality, Increases Marrow Adiposity and Alters Gut Microbiome in Mice.Foods (Basel, Switzerland) · 2021Article
- Preclinical models for investigating how bone marrow adipocytes influence bone and hematopoietic cellularity.Best practice & research. Clinical endocrinology & metabolism · 2021Review
- Exercise and Diet: Uncovering Prospective Mediators of Skeletal Fragility in Bone and Marrow Adipose Tissue.Current osteoporosis reports · 2020Review
- Adipocytes in hematopoiesis and acute leukemia: friends, enemies, or innocent bystanders?Leukemia · 2020Review
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
Abstract
Starvation induces low bone mass and high bone marrow adiposity in humans, but the underlying mechanisms are poorly understood. The adipokine leptin falls in starvation, suggesting that hypoleptinemia may be a link between negative energy balance, bone marrow fat accumulation, and impaired skeletal acquisition. In that case, treating mice with leptin during caloric restriction (CR) should reduce marrow adipose tissue (MAT) and improve bone mass. To test this hypothesis, female C57Bl/6J mice were fed a 30% CR or normal (N) diet from 5 to 10 weeks of age, with daily injections of vehicle (VEH), 1mg/kg leptin (LEP1), or 2mg/kg leptin (LEP2) (N=6-8/group). Outcomes included body mass, body fat percentage, and whole-body bone mineral density (BMD) via peripheral dual-energy X-ray absorptiometry, cortical and trabecular microarchitecture via microcomputed tomography (μCT), and MAT volume via μCT of osmium tetroxide-stained bones. Overall, CR mice had lower body mass, body fat percentage, BMD, and cortical bone area fraction, but more connected trabeculae, vs N mice (P<0.05 for all). Most significantly, although MAT was elevated in CR vs N overall, leptin treatment blunted MAT formation in CR mice by 50% vs VEH (P<0.05 for both leptin doses). CR LEP2 mice weighed less vs CR VEH mice at 9-10 weeks of age (P<0.05), but leptin treatment did not affect body fat percentage, BMD, or bone microarchitecture within either diet. These data demonstrate that once daily leptin bolus during CR inhibits bone marrow adipose expansion without affecting bone mass acquisition, suggesting that leptin has distinct effects on starvation-induced bone marrow fat formation and skeletal acquisition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.