ArticleThe EMBO journal2016
Structure of the frequency-interacting RNA helicase: a protein interaction hub for the circadian clock.
Article in The EMBO journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 39 citations in OpenAlex.
- Trends in circadian rhythms research in fungi since the millenium.Npj biological timing and sleep · 2026Review
- Core clock protein subcellular dynamics coordinate local and global circadian control in syncytia.The Journal of cell biology · 2026Article
- Disordered but rhythmic-the role of intrinsic protein disorder in eukaryotic circadian timing.FEBS letters · 2026Review
- Core Clock Protein Subcellular Dynamics Coordinate Local and Global Circadian Control in Syncytia.bioRxiv : the preprint server for biology · 2025Article
- Disordered clock protein interactions and charge blocks turn an hourglass into a persistent circadian oscillator.Nature communications · 2024Article
- Article
- The Function, Regulation, and Mechanism of Protein Turnover in Circadian Systems inInternational journal of molecular sciences · 2024Review
- Circadian regulation of physiology by disordered protein-protein interactions.Current opinion in structural biology · 2024Review
- The Frq-Frh Complex Light-Dependently Delays Sfl1-Induced Microsclerotia Formation inJournal of fungi (Basel, Switzerland) · 2023Article
- Differential regulation of phosphorylation, structure, and stability of circadian clock protein FRQ isoforms.The Journal of biological chemistry · 2023Article
- The Essential and the Nonessential Roles of Four Clock Elements in the Circadian Rhythm ofJournal of fungi (Basel, Switzerland) · 2022Article
- Hydrogen-deuterium exchange mass spectrometry of Mtr4 with diverse RNAs reveals substrate-dependent dynamics and interfaces in the arch.Nucleic acids research · 2022Article
- GIpred: a computational tool for prediction of GIGANTEA proteins using machine learning algorithm.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Using hydrogen-deuterium exchange mass spectrometry to characterize Mtr4 interactions with RNA.Methods in enzymology · 2022Article
- Circadian Interactomics: How Research Into Protein-Protein Interactions Beyond the Core Clock Has Influenced the Model of Circadian Timekeeping.Journal of biological rhythms · 2021Review
- Mtr4 RNA helicase structures and interactions.Biological chemistry · 2021Review
- Intrinsic disorder is an essential characteristic of components in the conserved circadian circuit.Cell communication and signaling : CCS · 2020Review
- Opposite Nuclear Dynamics of Two FRH-Dominated Frequency Proteins Orchestrate Non-Rhythmic Conidiation inCells · 2020Article
- NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction.Genes & development · 2019Article
- Nucleotide Spin Labeling for ESR Spectroscopy of ATP-Binding Proteins.Applied magnetic resonance · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
In the Neurospora crassa circadian clock, a protein complex of frequency (FRQ), casein kinase 1a (CK1a), and the FRQ-interacting RNA Helicase (FRH) rhythmically represses gene expression by the white-collar complex (WCC). FRH crystal structures in several conformations and bound to ADP/RNA reveal differences between FRH and the yeast homolog Mtr4 that clarify the distinct role of FRH in the clock. The FRQ-interacting region at the FRH N-terminus has variable structure in the absence of FRQ A known mutation that disrupts circadian rhythms (R806H) resides in a positively charged surface of the KOW domain, far removed from the helicase core. We show that changes to other similarly located residues modulate interactions with the WCC and FRQ A V142G substitution near the N-terminus also alters FRQ and WCC binding to FRH, but produces an unusual short clock period. These data support the assertion that FRH helicase activity does not play an essential role in the clock, but rather FRH acts to mediate contacts among FRQ, CK1a and the WCC through interactions involving its N-terminus and KOW module.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.