Evidence map›Paper›PMID 27339400›Full record

ArticleFEBS letters2016

Tetraspanin 8 mediates AEG-1-induced invasion and metastasis in hepatocellular carcinoma cells.

Maaged A Akiel, Prasanna K Santhekadur, Rachel G Mendoza, Ayesha Siddiq, Paul B Fisher, Devanand Sarkar

Abstract readLetter
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 29 citations in OpenAlex.

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  15. A Positive Tetraspanin 8 (TSPAN8)/β-Catenin Regulatory Loop Enhances the Stemness of Colorectal Cancer Cells.Medical science monitor : international medical journal of experimental and clinical research · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Maaged A AkielDepartments of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, VA, USA.ORCID 0000-0002-0211-2837
Prasanna K SanthekadurDepartments of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, VA, USA.
Rachel G MendozaDepartments of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, VA, USA.
Ayesha SiddiqDepartments of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, VA, USA.
Paul B FisherDepartments of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, VA, USA.
Devanand SarkarDepartments of Human and Molecular Genetics, Virginia Commonwealth University, Richmond, VA, USA.
Virginia Commonwealth University · US

Funding

VCU Neuroscience Center Core GrantP30NS047463 · NINDS · VIRGINIA COMMONWEALTH UNIVERSITY · PI POVLISHOCK, JOHN T · 2003 to 2013
$5.0M
A novel mediator of obesity associated hepatocellular carcinoma (HCC)R21CA183954 · NCI · VIRGINIA COMMONWEALTH UNIVERSITY · PI SARKAR, DEVANAND · 2014 to 2015
$365k
NCI NIH HHS R21 CA183954NINDS NIH HHS P30 NS047463
6 · The paper itself

Abstract

Astrocyte-elevated gene-1 (AEG-1) positively regulates tumor progression and metastasis. Here, we document that AEG-1 upregulates transcription of the membrane protein tetraspanin 8 (TSPAN8). Knocking down TSPAN8 in AEG-1-overexpressing human hepatocellular carcinoma (HCC) cells markedly inhibited invasion and migration without affecting proliferation. TSPAN8 knockdown profoundly abrogated AEG-1-induced primary tumor and intrahepatic metastasis in an orthopic xenograft model in athymic nude mice. Coculture of TSPAN8 knockdown cells with human umbilical vein endothelial cells (HUVEC) markedly inhibited HUVEC tube formation indicating that inhibition of angiogenesis might cause reduction in primary tumor size. TSPAN8 inhibition might be a potential therapeutic strategy for metastatic HCC.

Indexed as

AnimalsCarcinoma, HepatocellularCell Adhesion MoleculesCell Line, TumorCell ProliferationGene Expression Regulation, NeoplasticGene Knockdown TechniquesHumansHuman Umbilical Vein Endothelial CellsLiver NeoplasmsMembrane ProteinsMiceNeoplasm InvasivenessNeoplasm MetastasisRNA-Binding ProteinsTetraspaninsCell Adhesion MoleculesMembrane ProteinsMTDH protein, humanRNA-Binding ProteinsTetraspaninsTSPAN8 protein, humanangiogenesisastrocyte-elevated gene-1invasionmetastasistetraspanin 8

Identifiers

PMID27339400
PMCPMC4992437
OpenAlexW2501867035

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.