Evidence map›Paper›PMID 27338641›Full record

ArticleBiochemical and biophysical research communications2016

Transferring the C-terminus of the chemokine CCL21 to CCL19 confers enhanced heparin binding.

Austin J Barmore, Sally M Castex, Brittany A Gouletas, Alex J Griffith, Slater W Metz, Nicolas G Muelder, Michael J Populin, David M Sackett, Abigail M Schuster, Christopher T Veldkamp

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.6field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 21 citations in OpenAlex.

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  14. CCR7 Sulfotyrosine Enhances CCL21 Binding.International journal of molecular sciences · 2017
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Austin J BarmoreDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI 53190, USA.
Sally M CastexDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI 53190, USA.
Brittany A GouletasDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI 53190, USA.
Alex J GriffithDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI 53190, USA.
Slater W MetzDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI 53190, USA.
Nicolas G MuelderDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI 53190, USA.
Michael J PopulinDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI 53190, USA.
David M SackettDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI 53190, USA.
Abigail M SchusterDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI 53190, USA.
Christopher T VeldkampDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI 53190, USA; Department of Biochemistry, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA. Electronic address: veldkamc@uww.edu.
University of Wisconsin–Whitewater · US

Funding

Structural studies of chemokines that target metastatic cancer cells to the lymphR15CA159202 · NCI · UNIVERSITY OF WISCONSIN WHITEWATER · PI VELDKAMP, CHRISTOPHER T · 2011 to 2011
$335k
NCI NIH HHS R15 CA159202
6 · The paper itself

Abstract

Chemokines direct the migration of cells during various immune processes and are involved in many disease states. For example, CCL19 and CCL21, through activation of the CCR7 receptor, recruit dendritic cells and naïve T-cells to the secondary lymphoid organs aiding in balancing immune response and tolerance. However, CCL19 and CCL21 can also direct the metastasis of CCR7 expressing cancers. Chemokine binding to glycosaminoglycans, such as heparin, is as important to chemokine function as receptor activation. CCL21 is unique in that it contains an extended C-terminus not found in other chemokines like CCL19. Deletion of this extended C-terminus reduces CCL21's affinity for heparin and transferring the CCL21 C-terminus to CCL19 enhances heparin binding mainly through non-specific, electrostatic interactions.

Indexed as

Amino Acid SequenceChemokine CCL19Chemokine CCL21HeparinHumansNuclear Magnetic Resonance, BiomolecularProtein BindingSequence Homology, Amino AcidStatic ElectricityChemokine CCL19Chemokine CCL21HeparinCCL19CCL21ChemokinesChimeric proteinGlycosaminoglycansHeparin

Identifiers

PMID27338641
PMCPMC4967021
OpenAlexW2470852939

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.