Evidence map›Paper›PMID 27338587›Full record

ArticleMicrobiome2016

Reduced diversity and altered composition of the gut microbiome in individuals with myalgic encephalomyelitis/chronic fatigue syndrome.

Ludovic Giloteaux, Julia K Goodrich, William A Walters, Susan M Levine, Ruth E Ley, Maureen R Hanson

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiome, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 193 papers, 9 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
193citing papers in PubMed, 9 pooled it
18.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

193 citing papers in PubMed, 9 syntheses or guidelines pooled it, 358 citations in OpenAlex.

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  9. Dietary and nutrition interventions for the therapeutic treatment of chronic fatigue syndrome/myalgic encephalomyelitis: a systematic review.Journal of human nutrition and dietetics : the official journal of the British Dietetic Association · 2017
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133 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Ludovic GiloteauxDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.
Julia K GoodrichDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.
William A WaltersDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.
Susan M LevinePrivate Practice, New York, NY, USA.
Ruth E LeyDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.
Maureen R HansonDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA. mrh5@cornell.edu.
Cornell University · US

Funding

Microbiomes and Inflammation in Chronic Fatigue SyndromeR21AI101614 · NIAID · CORNELL UNIVERSITY · PI HANSON, MAUREEN REBECCA, LEY, RUTH EMILY · 2012 to 2013
$409k
NIAID NIH HHS R21 AI101614
6 · The paper itself

Abstract

backgroundGastrointestinal disturbances are among symptoms commonly reported by individuals diagnosed with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). However, whether ME/CFS is associated with an altered microbiome has remained uncertain. Here, we profiled gut microbial diversity by sequencing 16S ribosomal ribonucleic acid (rRNA) genes from stool as well as inflammatory markers from serum for cases (n = 48) and controls (n = 39). We also examined a set of inflammatory markers in blood: C-reactive protein (CRP), intestinal fatty acid-binding protein (I-FABP), lipopolysaccharide (LPS), LPS-binding protein (LBP), and soluble CD14 (sCD14).

resultsWe observed elevated levels of some blood markers for microbial translocation in ME/CFS patients; levels of LPS, LBP, and sCD14 were elevated in ME/CFS subjects. Levels of LBP correlated with LPS and sCD14 and LPS levels correlated with sCD14. Through deep sequencing of bacterial rRNA markers, we identified differences between the gut microbiomes of healthy individuals and patients with ME/CFS. We observed that bacterial diversity was decreased in the ME/CFS specimens compared to controls, in particular, a reduction in the relative abundance and diversity of members belonging to the Firmicutes phylum. In the patient cohort, we find less diversity as well as increases in specific species often reported to be pro-inflammatory species and reduction in species frequently described as anti-inflammatory. Using a machine learning approach trained on the data obtained from 16S rRNA and inflammatory markers, individuals were classified correctly as ME/CFS with a cross-validation accuracy of 82.93 %.

conclusionsOur results indicate dysbiosis of the gut microbiota in this disease and further suggest an increased incidence of microbial translocation, which may play a role in inflammatory symptoms in ME/CFS.

Indexed as

Acute-Phase ProteinsAdultAgedBacillotaBacteriaBiodiversityCarrier ProteinsCase-Control StudiesC-Reactive ProteinDNA, RibosomalDysbiosisFatigue Syndrome, ChronicFatty Acid-Binding ProteinsFecesFemaleGastrointestinal MicrobiomeAcute-Phase ProteinsCarrier ProteinsC-Reactive ProteinDNA, RibosomalFatty Acid-Binding ProteinsLipopolysaccharide-Binding ProteinLipopolysaccharide ReceptorsMembrane GlycoproteinsRNA, Ribosomal, 16SBeta-diversityChronic fatigue syndromeInflammationLipopolysaccharidesMicrobial translocationMicrobiomeMyalgic encephalomyelitis

Identifiers

PMID27338587
PMCPMC4918027
OpenAlexW2462189178

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.