ArticleMicroscopy research and technique2018
Selective plane illumination microscopy with a light sheet of uniform thickness formed by an electrically tunable lens.
Article in Microscopy research and technique, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Axially swept open-top light-sheet microscopy for densely labeled clinical specimens.Optics letters · 2024Article
- Interferometric modulation for generating extended light sheet: improving field of view.Journal of biomedical optics · 2024Article
- Light-Sheet Imaging to Reveal Cardiac Structure in Rodent Hearts.Journal of visualized experiments : JoVE · 2024Article
- Rapid 3D isotropic imaging of whole organ with double-ring light-sheet microscopy and self-learning side-lobe elimination.Biomedical optics express · 2023Article
- Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy.The Journal of cell biology · 2023Review
- An ensemble recruited by αNeuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Article
- BNST PKCδ neurons are activated by specific aversive conditions to promote anxiety-like behavior.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Article
- Motionless volumetric structured light sheet microscopy.Biomedical optics express · 2023Article
- Flow Cytometry with Anti-Diffraction Light Sheet (ADLS) by Spatial Light Modulation.Micromachines · 2023Article
- Organization of neural systems expressing melanocortin-3 receptors in the mouse brain: Evidence for sexual dimorphism.The Journal of comparative neurology · 2022Article
- Isotropic imaging across spatial scales with axially swept light-sheet microscopy.Nature protocols · 2022Review
- High contrast, isotropic, and uniform 3D-imaging of centimeter-scale scattering samples using structured illumination light-sheet microscopy with axial sweeping.Biomedical optics express · 2022Article
- CompassLSM: axially swept light-sheet microscopy made simple.Biomedical optics express · 2021Article
- Delineation of an insula-BNST circuit engaged by struggling behavior that regulates avoidance in mice.Nature communications · 2021Article
- Open-top axially swept light-sheet microscopy.Biomedical optics express · 2021Article
- Multi-Scale Light-Sheet Fluorescence Microscopy for Fast Whole Brain Imaging.Frontiers in neuroanatomy · 2021Article
- High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution.Journal of biomedical optics · 2020Article
- Lightsheet fluorescence lifetime imaging microscopy with wide-field time-correlated single photon counting.Journal of biophotonics · 2020Article
- Propagating-path uniformly scanned light sheet excitation microscopy for isotropic volumetric imaging of large specimens.Journal of biomedical optics · 2019Article
- Protection of tissue physicochemical properties using polyfunctional crosslinkers.Nature biotechnology · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Light sheet microscopy is a powerful technique for rapid, three-dimensional fluorescence imaging of large specimen such as drosophila and zebrafish embryos. Yet, beam divergence results in a loss of axial resolution at the periphery of the light sheet. Here, we demonstrate how an electrically tunable lens can be utilized to maintain the minimal, diffraction-limited thickness of the light sheet over a wide field of view (>600 µm) at high frame rates (40 fps). This mode of operation is necessary for the application of fluorescence fluctuation spectroscopy in images. Microsc. Res. Tech. 81:924-928, 2018. © 2016 Wiley Periodicals, Inc.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.