Evidence map›Paper›PMID 27338488›Full record

ArticleBiosensors2016

Rapid Detection of Food Allergens by Microfluidics ELISA-Based Optical Sensor.

Xuan Weng, Gautam Gaur, Suresh Neethirajan

Abstract read
In one paragraph

Article in Biosensors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it.

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  9. Enzyme-Linked Immunosorbent Assay: Types and Applications.Methods in molecular biology (Clifton, N.J.) · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Xuan WengBionano Lab, School of Engineering, University of Guelph, Guelph, ON N1G 2W1, Canada. xuanw@uoguelph.ca.
Gautam GaurBionano Lab, School of Engineering, University of Guelph, Guelph, ON N1G 2W1, Canada. ggaur@uoguelph.ca.
Suresh NeethirajanBionano Lab, School of Engineering, University of Guelph, Guelph, ON N1G 2W1, Canada. sneethir@uoguelph.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The risks associated with the presence of hidden allergens in food have increased the need for rapid, sensitive, and reliable methods for tracing food allergens in commodities. Conventional enzyme immunosorbent assay (ELISA) has usually been performed in a centralized lab, requiring considerable time and sample/reagent consumption and expensive detection instruments. In this study, a microfluidic ELISA platform combined with a custom-designed optical sensor was developed for the quantitative analysis of the proteins wheat gluten and Ara h 1. The developed microfluidic ELISA biosensor reduced the total assay time from hours (up to 3.5 h) to 15-20 min and decreased sample/reagent consumption to 5-10 μL, compared to a few hundred microliters in commercial ELISA kits, with superior sensitivity. The quantitative capability of the presented biosensor is a distinctive advantage over the commercially available rapid methods such as lateral flow devices (LFD) and dipstick tests. The developed microfluidic biosensor demonstrates the potential for sensitive and less-expensive on-site determination for rapidly detecting food allergens in a complex sample system.

Indexed as

Biosensing TechniquesEnzyme-Linked Immunosorbent AssayAllergensAntigens, PlantFoodGlutensGlycoproteinsLab-On-A-Chip DevicesMembrane ProteinsMicrofluidicsPlant ProteinsAllergensAntigens, PlantAra h 1 protein, Arachis hypogaeaGlutensGlycoproteinsMembrane ProteinsPlant ProteinsAra h 1food allergenglutenmicrofluidicsoptical sensor

Identifiers

PMID27338488
PMCPMC4931484

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.