ArticleInternational journal of molecular sciences2016
Toxicological Effects of Caco-2 Cells Following Short-Term and Long-Term Exposure to Ag Nanoparticles.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Impact of Milk on the Behavior and Toxicity of Nanosized SiOACS omega · 2025Article
- Group IB Metal-Based Nanomaterials for Antibacterial Applications.Small science · 2025Review
- Bioaccessibility and cellular transport study of silver and titanium dioxide nanoparticles from exposed seaweed and mussels using Caco-2 cells.Mikrochimica acta · 2025Article
- Assessing the Toxicity of Metal- and Carbon-Based Nanomaterials In Vitro: Impact on Respiratory, Intestinal, Skin, and Immune Cell Lines.International journal of molecular sciences · 2024Article
- An Adverse Outcome Pathway for food nanomaterial-induced intestinal barrier disruption.Frontiers in toxicology · 2024Article
- Article
- Identifying the Molecular Mechanisms and Types of Cell Death Induced byInternational journal of molecular sciences · 2022Article
- Repeated Exposure of Macrophages to Synthetic Amorphous Silica Induces Adaptive Proteome Changes and a Moderate Cell Activation.Nanomaterials (Basel, Switzerland) · 2022Article
- Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles.Molecules (Basel, Switzerland) · 2021Article
- Assessing the Toxicological Relevance of Nanomaterial Agglomerates and Aggregates Using Realistic Exposure In Vitro.Nanomaterials (Basel, Switzerland) · 2021Article
- Biotransformation of Silver Nanoparticles into Oro-Gastrointestinal Tract by Integrated In Vitro Testing Assay: Generation of Exposure-Dependent Physical Descriptors for Nanomaterial Grouping.Nanomaterials (Basel, Switzerland) · 2021Article
- A Low-Serum Culture System for ProlongedFrontiers in toxicology · 2021Review
- Proposing Urothelial and Muscle In Vitro Cell Models as a Novel Approach for Assessment of Long-Term Toxicity of Nanoparticles.International journal of molecular sciences · 2020Article
- An Integrated In Vitro-In Silico Approach for Silver Nanoparticle Dosimetry in Cell Cultures.Annals of biomedical engineering · 2020Article
- Mechanism of cell death induced by silica nanoparticles in hepatocyte cells is by apoptosis.International journal of molecular medicine · 2019Article
- The Bioavailability, Biodistribution, and Toxic Effects of Silica-Coated Upconversion NanoparticlesFrontiers in chemistry · 2019Article
- Copper Oxide Nanoparticles Cause a Dose-Dependent Toxicity via Inducing Reactive Oxygen Species in Drosophila.Nanomaterials (Basel, Switzerland) · 2018Article
- Evaluation of E. coli inhibition by plain and polymer-coated silver nanoparticles.Revista do Instituto de Medicina Tropical de Sao Paulo · 2018Article
- Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles.NPJ science of food · 2017Review
- Effects of Sub-lethal Concentrations of Silver Nanoparticles on a Simulated Intestinal Prokaryotic-Eukaryotic Interface.Frontiers in microbiology · 2017Article
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Extensive utilization increases the exposure of humans to Ag nanoparticles (NPs) via the oral pathway. To comprehensively address the action of Ag NPs to the gastrointestinal systems in real situations, i.e., the long-term low-dose exposure, we evaluated and compared the toxicity of three Ag NPs (20-30 nm with different surface coatings) to the human intestine cell Caco-2 after 1-day and 21-day exposures, using various biological assays. In both the short- and long-term exposures, the variety of surface coating predominated the toxicity of Ag NPs in a descending order of citrate-coated Ag NP (Ag-CIT), bare Ag NP (Ag-B), and poly (N-vinyl-2-pyrrolidone)-coated Ag NP (Ag-PVP). The short-term exposure induced cell growth inhibition and death. The cell viability loss appeared after cells were exposed to 0.7 μg/mL Ag-CIT, 0.9 μg/mL Ag-B or >1.0 μg/mL Ag-PVP for 24 h. The short-term and higher-dose exposure also induced reactive oxygen species (ROS) generation, mitochondrial damage, cell membrane leakage, apoptosis, and inflammation (IL-8 level). The long-term exposure only inhibited the cell proliferation. After 21-day exposure to 0.4 μg/mL Ag-CIT, the cell viability dropped to less than 50%, while cells exposed to 0.5 μg/mL Ag-PVP remained normal as the control. Generally, 0.3 μg/mL is the non-toxic dose for the long-term exposure of Caco-2 cells to Ag NPs in this study. However, cells presented inflammation after exposure to Ag NPs with the non-toxic dose in the long-term exposure.
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