ArticlePlant molecular biology2016
Copy number variation at the HvCBF4-HvCBF2 genomic segment is a major component of frost resistance in barley.
Article in Plant molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Multi-omics revolution to promote plant breeding efficiency.Frontiers in plant science · 2022Pooled it
- Genome-Wide Association Study (GWAS) for Freezing and De-Acclimation Tolerance in Polish Winter Barley.International journal of molecular sciences · 2026Article
- Impact of Alien Chromosome Introgression fromPlants (Basel, Switzerland) · 2025Article
- How Structural Variations Influence Crop Improvement.International journal of molecular sciences · 2025Review
- Translational insights into abiotic interactions: From Arabidopsis to crop plants.The Plant cell · 2025Review
- A barley pan-transcriptome reveals layers of genotype-dependent transcriptional complexity.Nature genetics · 2025Article
- Genome-wide identification and expression analysis of the CBF transcription factor family inPlant signaling & behavior · 2023Article
- TheCells · 2023Review
- Article
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- Extensive allele mining discovers novel genetic diversity in the loci controlling frost tolerance in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Genome-wide identification ofPeerJ · 2022Article
- The Relationships between Plant Developmental Traits and Winter Field Survival in Rye (Plants (Basel, Switzerland) · 2021Article
- The Breeding of Winter-Hardy Malting Barley.Plants (Basel, Switzerland) · 2021Review
- Article
- Applications of Multi-Omics Technologies for Crop Improvement.Frontiers in plant science · 2021Review
- Digital PCR: What Relevance to Plant Studies?Biology · 2020Review
- Segmental duplications are hot spots of copy number variants affecting barley gene content.The Plant journal : for cell and molecular biology · 2020Article
- Genome-wide identification and expression analysis of the CBF/DREB1 gene family in lettuce.Scientific reports · 2020Article
- Perspectives on Low Temperature Tolerance and Vernalization Sensitivity in Barley: Prospects for Facultative Growth Habit.Frontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A family of CBF transcription factors plays a major role in reconfiguring the plant transcriptome in response to low-freezing temperature in temperate cereals. In barley, more than 13 HvCBF genes map coincident with the major QTL FR-H2 suggesting them as candidates to explain the function of the locus. Variation in copy number (CNV) of specific HvCBFs was assayed in a panel of 41 barley genotypes using RT-qPCR. Taking advantage of an accurate phenotyping that combined Fv/Fm and field survival, resistance-associated variants within FR-H2 were identified. Genotypes with an increased copy number of HvCBF4 and HvCBF2 (at least ten and eight copies, respectively) showed greater frost resistance. A CAPS marker able to distinguish the CBF2A, CBF2B and CBF2A/B forms was developed and showed that all the higher-ranking genotypes in term of resistance harbour only CBF2A, while other resistant winter genotypes harbour also CBF2B, although at a lower CNV. In addition to the major involvement of the HvCBF4-HvCBF2 genomic segment in the proximal cluster of CBF elements, a negative role of HvCBF3 in the distal cluster was identified. Multiple linear regression models taking into account allelic variation at FR-H1/VRN-H1 explained 0.434 and 0.550 (both at p < 0.001) of the phenotypic variation for Fv/Fm and field survival respectively, while no interaction effect between CNV at the HvCBFs and FR-H1/VRN-H1 was found. Altogether our data suggest a major involvement of the CBF genes located in the proximal cluster, with no apparent involvement of the central cluster contrary to what was reported for wheat.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.