ArticleScientific reports2016
Systems pathway engineering of Corynebacterium crenatum for improved L-arginine production.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 63 citations in OpenAlex.
- Nitrogen Mediates Amino Acid Metabolism Affecting Stachydrine Synthesis inPlants (Basel, Switzerland) · 2026Article
- Enhancement and balance of 1,4-butanediamine biosynthesis in Escherichia coli based on modular metabolic engineering.Journal, genetic engineering & biotechnology · 2026Article
- Structure-based modeling and engineering of Corynebacterium glutamicum LysE transporter for efficient extrusion of L-arginine.Communications biology · 2025Article
- Rationally Engineering pH Adaptation of Acid-Induced Arginine Decarboxylase from Escherichia coli to Alkaline Environments to Efficiently Biosynthesize Putrescine.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- The flavohaemoprotein hmp maintains redox homeostasis in response to reactive oxygen and nitrogen species in Corynebacterium glutamicum.Microbial cell factories · 2023Article
- Reduction of acetate synthesis, enhanced arginine export, and supply of precursors, cofactors, and energy for improved synthesis of L-arginine by Escherichia coli.Applied microbiology and biotechnology · 2023Article
- Effective use of biosensors for high-throughput library screening for metabolite production.Journal of industrial microbiology & biotechnology · 2021Review
- Production of l-glutamate family amino acids inSynthetic and systems biotechnology · 2021Review
- Reconstruction of the Diaminopimelic Acid Pathway to Promote L-lysine Production inInternational journal of molecular sciences · 2021Article
- Phylogenomic Reappraisal of Fatty Acid Biosynthesis, Mycolic Acid Biosynthesis and Clinical Relevance Among Members of the GenusFrontiers in microbiology · 2021Article
- High-Quality Genome-Scale Reconstruction ofFrontiers in microbiology · 2021Article
- Diamine Biosynthesis: Research Progress and Application Prospects.Applied and environmental microbiology · 2020Review
- Rational modification of the carbon metabolism of Corynebacterium glutamicum to enhance L-leucine production.Journal of industrial microbiology & biotechnology · 2020Article
- Significantly enhancing production ofScience advances · 2020Article
- PII Signal Transduction Protein GlnK Alleviates Feedback Inhibition ofApplied and environmental microbiology · 2020Article
- Improvement of l-arginine production by in silico genome-scale metabolic network model guided genetic engineering.3 Biotech · 2020Article
- Development of a Novel Biosensor-Driven Mutation and Selection System viaFrontiers in bioengineering and biotechnology · 2020Article
- Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method.Microbial cell factories · 2019Article
- Enhancement of L-arginine production by increasing ammonium uptake in an AmtR-deficient Corynebacterium crenatum mutant.Journal of industrial microbiology & biotechnology · 2019Article
- Simultaneous Quantification of L-arginine and Monosaccharides during Fermentation: An Advanced Chromatography Approach.Molecules (Basel, Switzerland) · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
L-arginine is an important amino acid in food and pharmaceutical industries. Until now, the main production method of L-arginine in China is the highly polluting keratin acid hydrolysis. The industrial level L-arginine production by microbial fermentation has become an important task. In previous work, we obtained a new L-arginine producing Corynebacterium crenatum (subspecies of Corynebacterium glutamicum) through screening and mutation breeding. In this work, we performed systems pathway engineering of C. crenatum for improved L-arginine production, involving amplification of L-arginine biosynthetic pathway flux by removal of feedback inhibition and overexpression of arginine operon; optimization of NADPH supply by modulation of metabolic flux distribution between glycolysis and pentose phosphate pathway; increasing glucose consumption by strengthening the preexisting glucose transporter and exploitation of new glucose uptake system; channeling excess carbon flux from glycolysis into tricarboxylic acid cycle to alleviate the glucose overflow metabolism; redistribution of carbon flux at α-ketoglutarate metabolic node to channel more flux into L-arginine biosynthetic pathway; minimization of carbon and cofactor loss by attenuation of byproducts formation. The final strain could produce 87.3 g L(-1) L-arginine with yield up to 0.431 g L-arginine g(-1) glucose in fed-batch fermentation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.