ArticleClinical oral investigations2017
Effect of mouthwashes on the composition and metabolic activity of oral biofilms grown in vitro.
Article in Clinical oral investigations, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Effectiveness of mouthwashes on reducing SARS-CoV-2 viral load in oral cavity: a systematic review and meta-analysis.BMC oral health · 2023Pooled it
- An oxygen-releasing mouthwash shows species-dependent antibacterial and antibiofilm activity against periodontal pathogens.Clinical oral investigations · 2026Article
- An Oxygen-Releasing Mouthwash Reduces Porphyromonas gingivalis Biofilm and Suppresses fimA and hagA Expression.International dental journal · 2026Article
- Cost-Effectiveness of promoting preventive oral self-care in China: a population-based modelling study.BMJ public health · 2026Article
- The Effect of Chlorhexidine Mouthwashes on the Microbiota Associated with Peri-Implantitis Lesions: A Pilot Study.Antibiotics (Basel, Switzerland) · 2025Article
- 'Oxygen-based antiseptics' potential in the management of peri-implant mucositis.EXCLI journal · 2025Article
- Topical oxygen therapy as a novel strategy to promote wound healing and control the bacteria in implantology, oral surgery and periodontology: A review.The Saudi dental journal · 2024Review
- Effects of sodium lauryl sulfate and postbiotic toothpaste on oral microecology.Journal of oral microbiology · 2024Article
- The quorum quenching enzyme Aii20J modifiesFrontiers in cellular and infection microbiology · 2023Article
- Rapid Measurement of Lactate in the Exhaled Breath Condensate: Biosensor Optimization and In-Human Proof of Concept.ACS sensors · 2022Article
- Article
- Evidence on the Use of Mouthwash for the Control of Supragingival Biofilm and Its Potential Adverse Effects.Antibiotics (Basel, Switzerland) · 2022Review
- Ecological Effects of Daily Antiseptic Treatment on Microbial Composition of Saliva-Grown Microcosm Biofilms and Selection of Resistant Phenotypes.Frontiers in microbiology · 2022Article
- Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm.Scientific reports · 2021Article
- Potential prebiotic substrates modulate composition, metabolism, virulence and inflammatory potential of an in vitro multi-species oral biofilm.Journal of oral microbiology · 2021Article
- Protective effect of titanium tetrafluoride and silver diamine fluoride on radiation-induced dentin caries in vitro.Scientific reports · 2021Article
- Efficacy of Experimental Mouth Rinses on Caries-Related BiofilmsFrontiers in oral health · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe aim of this study was to determine the effect of an oxygenating mouthwash compared to two other established mouthwash products on bacterial composition and metabolic activity of oral biofilms in vitro. MATERIAL AND
methodsTwelve healthy subjects participated as donors. Plaque-saliva mixture inoculated biofilms were grown and treated with 3 different chemotherapeutic mouthwashes [amine fluoride/stannous fluoride (MD), oxygenating agent (AX), chlorhexidine 0.12 % (PA), and water (W)]. Effects of treatments were assessed on biofilm composition (16S rRNA gene amplicon sequencing), production of organic acids (formate, acetate, lactate, propionate, butyrate using capillary electrophoresis), and viability of the remaining biofilm (CFUs).
resultsMicrobial profiles of biofilms clustered per inoculum donor and were dominated by the genera Veillonella, Streptococcus, and Prevotella. Microbial diversity was only reduced after PA treatment. Significant changes in composition occurred after treatment with AX, resulting in lower proportions of Veillonella and higher proportions of non-mutans streptococci. Production of all organic acids after PA and lactate after MD was significantly lower as compared to W. AX resulted in reduction of acetate, butyrate, and propionate and increase in lactate production (p < 0.05). Viable counts were significantly lower after PA and AX treatments compared to W, while no significant reduction was observed after MD.
conclusionsAll studied mouthwashes affected the in vitro biofilms differently. The effects of the AX treatment were the most prominent which resulted in changes of the bacterial composition and metabolism. CLINICAL IMPLICATIONS: Awareness by the dental team that mouthwashes can change the bacterial composition and metabolism is important when advising its use.
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