ArticlePloS one2016
The Aberrant DNA Methylation Profile of Human Induced Pluripotent Stem Cells Is Connected to the Reprogramming Process and Is Normalized During In Vitro Culture.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- ICF1-Syndrome-AssociatedBiomolecules · 2023Article
- Maintenance of methylation profile in imprinting control regions in human induced pluripotent stem cells.Clinical epigenetics · 2022Article
- Epigenetic Mechanisms of ART-Related Imprinting Disorders: Lessons From iPSC and Mouse Models.Genes · 2021Review
- Modeling Maturity Onset Diabetes of the Young in Pluripotent Stem Cells: Challenges and Achievements.Frontiers in endocrinology · 2021Review
- Changes in Methylation Patterns of Tumor Suppressor Genes during Extended Human Embryonic Stem Cell Cultures.Stem cells international · 2021Article
- Tumorigenic and Immunogenic Properties of Induced Pluripotent Stem Cells: a Promising Cancer Vaccine.Stem cell reviews and reports · 2020Review
- Article
- Human in vitro models for understanding mechanisms of autism spectrum disorder.Molecular autism · 2020Review
- Endothelial Progenitor Cells Produced From Human Pluripotent Stem Cells by a Synergistic Combination of Cytokines, Small Compounds, and Serum-Free Medium.Frontiers in cell and developmental biology · 2020Article
- Epigenetic aberrations in human pluripotent stem cells.The EMBO journal · 2019Review
- High-efficiency derivation of human embryonic stem cell lines using a culture system with minimized trophoblast cell proliferation.Stem cell research & therapy · 2018Article
- Culture-induced recurrent epigenetic aberrations in human pluripotent stem cells.PLoS genetics · 2017Article
- Tumorigenic and Differentiation Potentials of Embryonic Stem Cells Depend on TGFStem cells international · 2017Article
- Human sensory neurons derived from pluripotent stem cells for disease modelling and personalized medicine.Neurobiology of pain (Cambridge, Mass.)Review
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Authors and funding
4 authors.
Funding
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Abstract
The potential clinical applications of human induced pluripotent stem cells (hiPSCs) are limited by genetic and epigenetic variations among hiPSC lines and the question of their equivalency with human embryonic stem cells (hESCs). We used MethylScreen technology to determine the DNA methylation profile of pluripotency and differentiation markers in hiPSC lines from different source cell types compared to hESCs and hiPSC source cells. After derivation, hiPSC lines compromised a heterogeneous population characterized by variable levels of aberrant DNA methylation. These aberrations were induced during somatic cell reprogramming and their levels were associated with the type of hiPSC source cells. hiPSC population heterogeneity was reduced during prolonged culture and hiPSCs acquired an hESC-like methylation profile. In contrast, the expression of differentiation marker genes in hiPSC lines remained distinguishable from that in hESCs. Taken together, in vitro culture facilitates hiPSC acquisition of hESC epigenetic characteristics. However, differences remain between both pluripotent stem cell types, which must be considered before their use in downstream applications.
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