ArticleBiomolecular detection and quantification2016
Development of NIST standard reference material 2373: Genomic DNA standards for HER2 measurements.
Article in Biomolecular detection and quantification, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Gene Copy Number Dictates Extracellular Vesicle Cargo.International journal of molecular sciences · 2025Article
- Digital PCR Validation for Characterization of Quantitative Reference Material ofMethods and protocols · 2024Article
- Highly sensitive droplet digital PCR for detection of RET fusion in papillary thyroid cancer.BMC cancer · 2023Article
- Multiplex Droplet Digital PCR Assay for Detection ofCancers · 2022Article
- Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements.Analytical and bioanalytical chemistry · 2022Review
- Lentiviral vector-based xenograft tumors as candidate reference materials for detection of HER2-low breast cancer.Frontiers in oncology · 2022Article
- Development and interlaboratory evaluation of a NIST Reference Material RM 8366 for EGFR and MET gene copy number measurements.Clinical chemistry and laboratory medicine · 2019Article
- A model for the impact of FFPE section thickness on gene copy number measurement by FISH.Scientific reports · 2019Article
- Cellular Metrology: Scoping for a Value Proposition in Extra- and Intracellular Measurements.Frontiers in bioengineering and biotechnology · 2019Review
- Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples.BioTechniques · 2018Article
- Single-day HER2neu amplification assessment using chip-based digital PCR in formalin-fixed paraffin-embedded breast carcinoma tissue.Breast cancer (Dove Medical Press) · 2018Article
- Analytic Response Curves of Clinical Breast Cancer IHC Tests.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2017Article
- Certified DNA Reference Materials to Compare HER2 Gene Amplification Measurements Using Next-Generation Sequencing Methods.The Journal of molecular diagnostics : JMD · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NIST standard reference material (SRM) 2373 was developed to improve the measurements of the HER2 gene amplification in DNA samples. SRM 2373 consists of genomic DNA extracted from five breast cancer cell lines with different amounts of amplification of the HER2 gene. The five components are derived from the human cell lines SK-BR-3, MDA-MB-231, MDA-MB-361, MDA-MB-453, and BT-474. The certified values are the ratios of the HER2 gene copy numbers to the copy numbers of selected reference genes DCK, EIF5B, RPS27A, and PMM1. The ratios were measured using quantitative polymerase chain reaction and digital PCR, methods that gave similar ratios. The five components of SRM 2373 have certified HER2 amplification ratios that range from 1.3 to 17.7. The stability and homogeneity of the reference materials were shown by repeated measurements over a period of several years. SRM 2373 is a well characterized genomic DNA reference material that can be used to improve the confidence of the measurements of HER2 gene copy number.
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