ArticleF1000Research2016
Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle.
Article in F1000Research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 12 citations in OpenAlex.
- Smooth Muscle-Alpha Actin R149C Pathogenic Variant Downregulates Integrin Recruitment at Cell-Matrix Adhesions and Decreases Cellular Contractility.International journal of molecular sciences · 2023Article
- A deep learning approach to identify and segment alpha-smooth muscle actin stress fiber positive cells.Scientific reports · 2021Article
- Effects of Low and High Aneurysmal Wall Shear Stress on Endothelial Cell Behavior: Differences and Similarities.Frontiers in physiology · 2021Article
- Myofibroblast Markers and Microscopy Detection Methods in Cell Culture and Histology.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Loss of smooth muscle α-actin effects on mechanosensing and cell-matrix adhesions.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- CAP2 deficiency delays myofibril actin cytoskeleton differentiation and disturbs skeletal muscle architecture and function.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Metabolic Reprogramming Promotes Myogenesis During Aging.Frontiers in physiology · 2019Article
- Expression of α-smooth muscle actin in the periodontal ligament during post-emergent tooth eruption.The Journal of international medical research · 2018Article
- Vascular Smooth Muscle Contractile Function Declines With Age in Skeletal Muscle Feed Arteries.Frontiers in physiology · 2018Article
- Actin isoform expression patterns in adult extracardiac and cardiac rhabdomyomas indicate a different cell of origin.Virchows Archiv : an international journal of pathology · 2017Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Higher vertebrates (mammals and birds) express six different highly conserved actin isoforms that can be classified in three subgroups: 1) sarcomeric actins, α-skeletal (α-SKA) and α-cardiac (α-CAA), 2) smooth muscle actins (SMAs), α-SMA and γ-SMA, and 3) cytoplasmic actins (CYAs), β-CYA and γ-CYA. The variations among isoactins, in each subgroup, are due to 3-4 amino acid differences located in their acetylated N-decapeptide sequence. The first monoclonal antibody (mAb) against an actin isoform (α-SMA) was produced and characterized in our laboratory in 1986 (Skalli et al., 1986) . We have further obtained mAbs against the 5 other isoforms. In this report, we focus on the mAbs anti-α-SKA and anti-α-CAA obtained after immunization of mice with the respective acetylated N-terminal decapeptides using the Repetitive Immunizations at Multiple Sites Strategy (RIMMS). In addition to the identification of their epitope by immunoblotting, we describe the expression of the 2 sarcomeric actins in mature skeletal muscle and during muscle repair after micro-lesions. In particular, we analyze the expression of α-CAA, α-SKA and α-SMA by co-immunostaining in a time course frame during the muscle repair process. Our results indicate that a restricted myocyte population expresses α-CAA and suggest a high capacity of self-regeneration in muscle cells. These antibodies may represent a helpful tool for the follow-up of muscle regeneration and pathological changes.
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