Evidence map›Paper›PMID 27335584›Full record

ArticleChemistry Central journal2016

A simple and convenient method for the preparation of antioxidant peptides from walnut (Juglans regia L.) protein hydrolysates.

Ming-Chuan Liu, Sheng-Jie Yang, Da Hong, Jin-Ping Yang, Min Liu, Yun Lin, Chia-Hui Huang, Chao-Jih Wang

Open access · goldAbstract read
In one paragraph

Article in Chemistry Central journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 51 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Article
  7. Article
  8. Review
  9. Article
  10. Quantitative In Silico Evaluation of Allergenic Proteins fromCurrent issues in molecular biology · 2022
    Article
  11. Review
  12. Bioactive Peptides from Walnut Residue Protein.Molecules (Basel, Switzerland) · 2020
    Review
  13. Article
  14. Article
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Ming-Chuan LiuR&D Center, Sinphar Tian-Li Pharmaceutical Co., Ltd., Hangzhou, 311100 China.
Sheng-Jie YangR&D Center, Sinphar Tian-Li Pharmaceutical Co., Ltd., Hangzhou, 311100 China.
Da HongR&D Center, Sinphar Tian-Li Pharmaceutical Co., Ltd., Hangzhou, 311100 China.
Jin-Ping YangR&D Center, Sinphar Tian-Li Pharmaceutical Co., Ltd., Hangzhou, 311100 China.
Min LiuR&D Center, Sinphar Tian-Li Pharmaceutical Co., Ltd., Hangzhou, 311100 China.
Yun LinR&D Center, Sinphar Tian-Li Pharmaceutical Co., Ltd., Hangzhou, 311100 China.
Chia-Hui HuangR&D Center, Sinphar Tian-Li Pharmaceutical Co., Ltd., Hangzhou, 311100 China.
Chao-Jih WangR&D Center, Sinphar Tian-Li Pharmaceutical Co., Ltd., Hangzhou, 311100 China ; School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical Univerisity, Shenyang, 110016 China ; R&D Center, Sinphar Pharmaceutical Co., Ltd., Ilan (Taiwan), 269 China.
Shenyang Pharmaceutical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundWalnut (Juglans regia L.), that belongs to the Juglandaceae family, is one of the nuts commonly found in Chinese diets. Researchers had obtained peptides from walnut protein hydrolysates, and these peptides exhibited the high antioxidant activities. The objective of this study was to develop a simple and convenient method for a facile and reproducible preparation of antioxidant peptides from walnut protein hydrolysates.

resultsWalnut proteins were extracted from walnut kernels using continuous countercurrent extraction process, and were separately hydrolyzed with six types of proteases (neutrase, papain, bromelain, alcalase, pepsin, and pancreatin). Then, hydrolysates were purified by ultrafiltration. The yields and purity of the peptides prepared using neutrase and papain were 16 and 81 % at least, respectively, higher than others, and had low molecular weight, 99 % of which were less than 1500 Da. Furthermore, the bioassay indicated that the two peptides exhibited the high antioxidant activities in the DPPH (IC50 values: 59.40 and 31.02 µg/mL, respectively), ABTS (IC50 values: 80.36 and 62.22 µg/mL, respectively), and superoxide radical scavenging assay (IC50 values: 107.47 and 80.00 µg/mL, respectively).

conclusionsThe method combines the advantages of generality, rapidity, simplicity, and is useful for the mass production of walnut peptides.Graphical AbstractPreparation of antioxidant peptides from walnut (Juglans regia L.) protein hydrolysates.

Indexed as

AntioxidantLarge scale preparationPeptideProteasesProteinWalnut

Identifiers

PMID27335584
PMCPMC4916535
OpenAlexW2467072189

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.