ArticleProceedings of the National Academy of Sciences of the United States of America2016
Evidence for a cysteine-mediated mechanism of excitation energy regulation in a photosynthetic antenna complex.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 51 citations in OpenAlex.
- Theoretical Study on the Photophysical Mechanism for the Detection of Biothiols Based on the 1,3,4-Thiadiazole Fluorescence Probe.Journal of fluorescence · 2026Article
- Dissipation Pathways in a Photosynthetic Complex.The journal of physical chemistry letters · 2025Article
- Modulation of singlet excited state dynamics of bacteriochlorophyll a in Fenna-Matthews-Olson (FMO) complex by PscB, a subunit with an intrinsically disordered region in Chlorobaculum tepidum reaction center.Photosynthesis research · 2025Article
- Phylogenetic and spectroscopic insights on the evolution of core antenna proteins in cyanobacteria.Photosynthesis research · 2024Article
- Living on the edge: light-harvesting efficiency and photoprotection in the core of green sulfur bacteria.Physical chemistry chemical physics : PCCP · 2023Article
- Determining interchromophore effects for energy transport in molecular networks using machine-learning algorithms.Physical chemistry chemical physics : PCCP · 2023Article
- Redox conditions correlated with vibronic coupling modulate quantum beats in photosynthetic pigment-protein complexes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Recent advances in the structural diversity of reaction centers.Photosynthesis research · 2021Article
- Photosynthesis tunes quantum-mechanical mixing of electronic and vibrational states to steer exciton energy transfer.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A Novel Mode of Photoprotection Mediated by a Cysteine Residue in the Chlorophyll Protein IsiA.mBio · 2021Article
- Neutron and X-ray analysis of the Fenna-Matthews-Olson photosynthetic antenna complex from Prosthecochloris aestuarii.Acta crystallographica. Section F, Structural biology communications · 2019Article
- Physiological Studies ofmBio · 2018Article
- Remembering John M. Olson (1929-2017).Photosynthesis research · 2018Article
- Photoprotective, excited-state quenching mechanisms in diverse photosynthetic organisms.The Journal of biological chemistry · 2018Review
- Cysteine-mediated mechanism disrupts energy transfer to prevent photooxidation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
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8 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Light-harvesting antenna complexes not only aid in the capture of solar energy for photosynthesis, but regulate the quantity of transferred energy as well. Light-harvesting regulation is important for protecting reaction center complexes from overexcitation, generation of reactive oxygen species, and metabolic overload. Usually, this regulation is controlled by the association of light-harvesting antennas with accessory quenchers such as carotenoids. One antenna complex, the Fenna-Matthews-Olson (FMO) antenna protein from green sulfur bacteria, completely lacks carotenoids and other known accessory quenchers. Nonetheless, the FMO protein is able to quench energy transfer in aerobic conditions effectively, indicating a previously unidentified type of regulatory mechanism. Through de novo sequencing MS, chemical modification, and mutagenesis, we have pinpointed the source of the quenching action to cysteine residues (Cys49 and Cys353) situated near two low-energy bacteriochlorophylls in the FMO protein from Chlorobaculum tepidum Removal of these cysteines (particularly removal of the completely conserved Cys353) through N-ethylmaleimide modification or mutagenesis to alanine abolishes the aerobic quenching effect. Electrochemical analysis and electron paramagnetic resonance spectra suggest that in aerobic conditions the cysteine thiols are converted to thiyl radicals which then are capable of quenching bacteriochlorophyll excited states through electron transfer photochemistry. This simple mechanism has implications for the design of bio-inspired light-harvesting antennas and the redesign of natural photosynthetic systems.
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