ArticleThe Plant cell2016
Multiple Calmodulin-Binding Sites Positively and Negatively Regulate Arabidopsis CYCLIC NUCLEOTIDE-GATED CHANNEL12.
Article in The Plant cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Calcium-ROS-NO signalling integration in plant immunity: mechanistic interactions and feedback regulation.Planta · 2026Review
- Proteome-Wide Prediction of Putative Bemisia tabaci Effector Candidates and Transient Validation of BtApe-Mediated Suppression of Tomato Immune Responses.Archives of insect biochemistry and physiology · 2026Article
- miRNA-CNGC/GLR circuits coordinate heat-stress CaProtoplasma · 2026Article
- TaCNGC-2A suppresses seed dormancy and activates pre-harvest sprouting through modulating calcium and hormonal signaling pathways.Nature communications · 2026Article
- A BRI1-CNGC12 phosphorylation module links hormone signaling to manganese homeostasis in plants.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Cyclic nucleotide-gated channels serve as core components for CaJournal of plant research · 2026Article
- Transcriptomic Analyses of Tomato Exhibiting Induced Resistance toPlants (Basel, Switzerland) · 2025Article
- Hydrogen sulfide promotes lateral root formation in peach through persulfidation of SnRK1α kinase.Plant biotechnology journal · 2025Article
- BrCNGC12 and BrCNGC16 mediate CaPlant biotechnology journal · 2025Article
- Genome-Wide Identification and Expression Analysis of thePlants (Basel, Switzerland) · 2024Article
- Genome-Wide Identification and Expression Analysis of the Cyclic Nucleotide-Gated Channel Gene Family inInternational journal of molecular sciences · 2024Article
- OPEN STOMATA 1 phosphorylates CYCLIC NUCLEOTIDE-GATED CHANNELs to trigger Ca2+ signaling for abscisic acid-induced stomatal closure in Arabidopsis.The Plant cell · 2024Article
- A charged existence: A century of transmembrane ion transport in plants.Plant physiology · 2024Review
- Long small RNA76113 targets CYCLIC NUCLEOTIDE-GATED ION CHANNEL 5 to repress disease resistance in rice.Plant physiology · 2024Article
- Calcium Signaling and the Response to Heat Shock in Crop Plants.International journal of molecular sciences · 2023Review
- Genome-wide identification and expression analysis of the cyclic nucleotide-gated ion channel (CNGC) gene family in Saccharum spontaneum.BMC genomics · 2023Article
- Genome-Wide Identification, Characterization and Experimental Expression Analysis of CNGC Gene Family inInternational journal of molecular sciences · 2023Article
- Multiple cyclic nucleotide-gated channels function as ABA-activated Ca2+ channels required for ABA-induced stomatal closure in Arabidopsis.The Plant cell · 2023Article
- Article
- Calcium Signaling in Plant-Insect Interactions.Plants (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
8 authors.
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Abstract
Ca(2+) signaling is critical to plant immunity; however, the channels involved are poorly characterized. Cyclic nucleotide-gated channels (CNGCs) are nonspecific, Ca(2+)-permeable cation channels. Plant CNGCs are hypothesized to be negatively regulated by the Ca(2+) sensor calmodulin (CaM), and previous work has focused on a C-terminal CaM-binding domain (CaMBD) overlapping with the cyclic nucleotide binding domain of plant CNGCs. However, we show that the Arabidopsis thaliana isoform CNGC12 possesses multiple CaMBDs at cytosolic N and C termini, which is reminiscent of animal CNGCs and unlike any plant channel studied to date. Biophysical characterizations of these sites suggest that apoCaM interacts with a conserved isoleucine-glutamine (IQ) motif in the C terminus of the channel, while Ca(2+)/CaM binds additional N- and C-terminal motifs with different affinities. Expression of CNGC12 with a nonfunctional N-terminal CaMBD constitutively induced programmed cell death, providing in planta evidence of allosteric CNGC regulation by CaM. Furthermore, we determined that CaM binding to the IQ motif was required for channel function, indicating that CaM can both positively and negatively regulate CNGC12. These data indicate a complex mode of plant CNGC regulation by CaM, in contrast to the previously proposed competitive ligand model, and suggest exciting parallels between plant and animal channels.
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