Evidence map›Paper›PMID 27335152›Full record

ArticleJournal of clinical microbiology2016

Spoligotyping of Mycobacterium tuberculosis Complex Isolates by Use of Ligation-Based Amplification and Melting Curve Analysis.

Xiaohong Zeng, Hui Li, Rongrong Zheng, Natalia Kurepina, Barry N Kreiswirth, Xilin Zhao, Ye Xu, Qingge Li

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Molecular epidemiology and genetic diversity of Mycobacterium tuberculosis complex in referral health centers of Bamako, Mali: What is new?International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2022
    Article
  8. Article
  9. Microorganisms · 2020
    Review
  10. Rapid Identification of Clinically RelevantJournal of clinical microbiology · 2019
    Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xiaohong ZengEngineering Research Centre of Molecular Diagnostics, Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, China State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, China Engineering Research Centre of Personalized Molecular Diagnostics of Xiamen, Fujian, China.
Hui LiTuberculosis Reference Laboratory, Henan Center for Disease Control and Prevention, Zhengzhou, Henan, China.
Rongrong ZhengDivision of Tuberculosis Control and Prevention, Xiamen Center for Disease Control and Prevention, Xiamen, Fujian, China.
Natalia KurepinaPublic Health Research Institute, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
Barry N KreiswirthPublic Health Research Institute, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
Xilin ZhaoState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, China Public Health Research Institute, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
Ye XuEngineering Research Centre of Molecular Diagnostics, Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, China State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, China Engineering Research Centre of Personalized Molecular Diagnostics of Xiamen, Fujian, China xuye@xmu.edu.cn qgli@xmu.edu.cn.
Qingge LiEngineering Research Centre of Molecular Diagnostics, Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, China State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, China Engineering Research Centre of Personalized Molecular Diagnostics of Xiamen, Fujian, China xuye@xmu.edu.cn qgli@xmu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We report here a ligation-based spoligotyping that can identify unamplified spacers in membrane-based spoligotyping due to asymmetric insertion of IS6110 in the direct repeat locus. Our typing yielded 84.4% (411/487) concordance with traditional typing and 100% (487/487) accuracy when confirmed by DNA sequencing.

Indexed as

Transition TemperatureDNA, BacterialDNA Transposable ElementsMolecular TypingMycobacterium tuberculosisNucleic Acid Amplification TechniquesRepetitive Sequences, Nucleic AcidDNA, BacterialDNA Transposable Elements

Identifiers

PMID27335152
PMCPMC5005503

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.