ArticleJournal of nanobiotechnology2016
Designing, construction and characterization of genetically encoded FRET-based nanosensor for real time monitoring of lysine flux in living cells.
Article in Journal of nanobiotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 53 citations in OpenAlex.
- Recent advances in screening amino acid overproducers.Engineering microbiology · 2023Review
- Developments in FRET- and BRET-Based Biosensors.Micromachines · 2022Review
- Effective use of biosensors for high-throughput library screening for metabolite production.Journal of industrial microbiology & biotechnology · 2021Review
- Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology.Plant physiology · 2021Review
- FRET-Based Genetically Encoded Nanosensor for Real-Time Monitoring of the Flux of α-Tocopherol in Living Cells.ACS omega · 2021Article
- Considerations for using isolated cell systems to understand cardiac metabolism and biology.Journal of molecular and cellular cardiology · 2021Review
- Imaging Approaches for the Study of Metabolism in Real Time Using Genetically Encoded Reporters.Frontiers in cell and developmental biology · 2021Review
- Designing and Development of FRET-Based Nanosensor for Real Time Analysis of N-Acetyl-5-Neuraminic Acid in Living Cells.Frontiers in nutrition · 2021Article
- The Role of Amino Acids in Neurotransmission and Fluorescent Tools for Their Detection.International journal of molecular sciences · 2020Review
- A Non-Invasive Tool for Real-Time Measurement of Sulfate in Living Cells.International journal of molecular sciences · 2020Article
- Metabolic Flux Analysis of Catechin Biosynthesis Pathways Using Nanosensor.Antioxidants (Basel, Switzerland) · 2020Article
- Enhanced sensitivity and detection range of FRET-based vitamin B3 Biotech · 2020Article
- Real-Time Optical Detection of Isoleucine in Living Cells through a Genetically-Encoded Nanosensor.Sensors (Basel, Switzerland) · 2019Article
- Synthesis of eco-friendly silver nanoparticles usingSaudi journal of biological sciences · 2019Article
- FRET Microscopy in Yeast.Biosensors · 2019Review
- Engineering genetically encoded FRET-based nanosensors for real time display of arsenic (AsScientific reports · 2019Article
- Conversion of Cytochrome P450 2D6 of Human Into a FRET-Based Tool for Real-Time Monitoring of Ajmalicine in Living Cells.Frontiers in bioengineering and biotechnology · 2019Article
- Role of green fluorescent proteins and their variants in development of FRET-based sensors.Journal of biosciences · 2018Review
- In vivo biosensors: mechanisms, development, and applications.Journal of industrial microbiology & biotechnology · 2018Article
- Nanoscale gizmos - the novel fluorescent probes for monitoring protein activity.Biochemical engineering journal · 2018Review
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEngineering microorganisms in order to improve the metabolite flux needs a detailed knowledge of the concentrations and flux rates of metabolites and metabolic intermediates in vivo. Fluorescence resonance energy transfer (FRET) based genetically encoded nanosensors represent a promising tool for measuring the metabolite levels and corresponding rate changes in live cells. Here, we report the development of a series of FRET based genetically encoded nanosensor for real time measurement of lysine at cellular level, as the improvement of microbial strains for the production of L-lysine is of major interest in industrial biotechnology.
resultsThe lysine binding periplasmic protein (LAO) from Salmonella enterica serovar typhimurium LT2 strain was used as the reporter element for the sensor. The LAO was sandwiched between GFP variants i.e. cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP). Affinity, pH stability, specificity and metal ions effects was scrutinized for the in vitro characterization of this nanosensor, named as FLIPK. The FLIPK is specific to lysine and found to be stable with the pH within the physiological range. The calculated affinity (K d ) of FLIPK was 97 µM. For physiological applications, mutants with different binding affinities were also generated and investigated in vitro. The developed nanosensor efficiently monitored the intracellular level of lysine in bacterial as well as yeast cell.
conclusionThe developed novel lysine fluorescence resonance energy transfer sensors can be used for in vivo monitoring of lysine levels in prokaryotes as well as eukaryotes. The potential of these sensors is that they can be used as reporter tools in the development of metabolically engineered microbial strains or for real-time monitoring of intracellular lysine during fermentation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.