ArticleJournal of nanobiotechnology2016
Quantification and visualization of cellular uptake of TiO2 and Ag nanoparticles: comparison of different ICP-MS techniques.
Article in Journal of nanobiotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 104 citations in OpenAlex.
- Quantitative LA-ICP-MS Imaging of Elemental Distributions with Protein Correlation in 3D Tumor Models.Analytical chemistry · 2026Article
- Harnessing ICP-MS for Quantitative Analysis of Metal-Based Nanoparticle-Cell Interactions.Advances in experimental medicine and biology · 2026Review
- The nano-paradox: addressing nanotoxicity for sustainable agriculture, circular economy and SDGs.Journal of nanobiotechnology · 2025Review
- Single cell-inductively coupled plasma-mass spectrometry (SC-ICP-MS) reveals metallic heterogeneity in a macrophage model of infectious diseases.Analytical and bioanalytical chemistry · 2024Article
- Toxicity and magnetometry evaluation of the uptake of core-shell maghemite-silica nanoparticles by neuroblastoma cells.Royal Society open science · 2024Article
- Expanding the boundaries of atomic spectroscopy at the single-cell level: critical review of SP-ICP-MS, LIBS and LA-ICP-MS advances for the elemental analysis of tissues and single cells.Analytical and bioanalytical chemistry · 2023Review
- Effect of Exposure Concentration and Growth Conditions on the Association of Cerium Oxide Nanoparticles with Green Algae.Nanomaterials (Basel, Switzerland) · 2023Article
- High-Throughput Single-Cell Analysis of Nanoparticle-Cell Interactions.Trends in analytical chemistry : TRAC · 2023Article
- Investigating the Cellular Uptake of Model Nanoplastics by Single-Cell ICP-MS.Nanomaterials (Basel, Switzerland) · 2023Article
- Glycocalyx Components Detune the Cellular Uptake of Gold Nanoparticles in a Size- and Charge-Dependent Manner.ACS applied bio materials · 2023Article
- Facets of ICP-MS and their potential in the medical sciences-Part 2: nanomedicine, immunochemistry, mass cytometry, and bioassays.Analytical and bioanalytical chemistry · 2022Review
- Distinct Uptake Routes Participate in Silver Nanoparticle Engulfment by Earthworm and Human Immune Cells.Nanomaterials (Basel, Switzerland) · 2022Article
- Article
- Multiplexed Analysis of the Cellular Uptake of Polymeric Nanocarriers.Analytical chemistry · 2022Article
- Silver Micro-Nanoparticle-Based Nanoarchitectures: Synthesis Routes, Biomedical Applications, and Mechanisms of Action.Polymers · 2021Review
- Cellular binding, uptake and biotransformation of silver nanoparticles in human T lymphocytes.Nature nanotechnology · 2021Article
- A Sensitive Single Particle-ICP-MS Method for CeOJournal of agricultural and food chemistry · 2021Article
- Treatment of Breast Cancer-Bearing BALB/c Mice with Magnetic Hyperthermia using Dendrimer Functionalized Iron-Oxide Nanoparticles.Nanomaterials (Basel, Switzerland) · 2020Article
- Suitability of 3D human brain spheroid models to distinguish toxic effects of gold and poly-lactic acid nanoparticles to assess biocompatibility for brain drug delivery.Particle and fibre toxicology · 2019Article
- Endocytosis, intracellular fate, accumulation, and agglomeration of titanium dioxide (TiOEnvironmental science and pollution research international · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSafety assessment of nanoparticles (NPs) requires techniques that are suitable to quantify tissue and cellular uptake of NPs. The most commonly applied techniques for this purpose are based on inductively coupled plasma mass spectrometry (ICP-MS). Here we apply and compare three different ICP-MS methods to investigate the cellular uptake of TiO2 (diameter 7 or 20 nm, respectively) and Ag (diameter 50 or 75 nm, respectively) NPs into differentiated mouse neuroblastoma cells (Neuro-2a cells). Cells were incubated with different amounts of the NPs. Thereafter they were either directly analyzed by laser ablation ICP-MS (LA-ICP-MS) or were lysed and lysates were analyzed by ICP-MS and by single particle ICP-MS (SP-ICP-MS).
resultsAll techniques confirmed that smaller particles were taken up to a higher extent when values were converted in an NP number-based dose metric. In contrast to ICP-MS and LA-ICP-MS, this measure is already directly provided through SP-ICP-MS. Analysis of NP size distribution in cell lysates by SP-ICP-MS indicates the formation of NP agglomerates inside cells. LA-ICP-MS imaging shows that some of the 75 nm Ag NPs seemed to be adsorbed onto the cell membranes and were not penetrating into the cells, while most of the 50 nm Ag NPs were internalized. LA-ICP-MS confirms high cell-to-cell variability for NP uptake.
conclusionsBased on our data we propose to combine different ICP-MS techniques in order to reliably determine the average NP mass and number concentrations, NP sizes and size distribution patterns as well as cell-to-cell variations in NP uptake and intracellular localization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.