ArticlePLoS pathogens2016
The WOPR Protein Ros1 Is a Master Regulator of Sporogenesis and Late Effector Gene Expression in the Maize Pathogen Ustilago maydis.
Article in PLoS pathogens, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 104 citations in OpenAlex.
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- "Select and Resequence" Methods Enable a Genome-Wide Association Study of the Dimorphic Human Fungal Pathogen Coccidioides posadasii.Genome biology and evolution · 2025Article
- Transcriptomic atlas throughout Coccidioides development reveals key phase-enriched transcripts of this important fungal pathogen.PLoS biology · 2025Article
- The SUbventral-Gland Regulator (SUGR-1) of nematode virulence.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- MaPac2, a Transcriptional Regulator, Is Involved in Conidiation, Stress Tolerances and Pathogenicity inJournal of fungi (Basel, Switzerland) · 2025Article
- Specific Transcriptional Regulation Controls Plant Organ-Specific Infection by the Oomycete Pathogen Phytophthora sojae.Molecular plant pathology · 2024Article
- Role ofJournal of fungi (Basel, Switzerland) · 2024Article
- Transcriptomic atlas of the morphologic development of the fungal pathogenbioRxiv : the preprint server for biology · 2024Article
- Inferring the composition of a mixed culture of natural microbial isolates by deep sequencing.bioRxiv : the preprint server for biology · 2024Article
- Functional identification of DNA demethylase geneFrontiers in plant science · 2024Article
- Characterization of Arbuscular Mycorrhizal Effector Proteins.International journal of molecular sciences · 2023Review
- Progress in pathogenesis research of Ustilago maydis, and the metabolites involved along with their biosynthesis.Molecular plant pathology · 2023Review
- Rgs1 is a regulator of effector gene expression during plant infection by the rice blast fungusProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Diploid mycelia ofFrontiers in microbiology · 2023Article
- Coccidioides Species: A Review of Basic Research: 2022.Journal of fungi (Basel, Switzerland) · 2022Review
- Decoding Transcription Regulatory Mechanisms Associated withmSystems · 2022Article
- Heat Shock Transcription Factor CgHSF1 Is Required for Melanin Biosynthesis, Appressorium Formation, and Pathogenicity inJournal of fungi (Basel, Switzerland) · 2022Article
- Construction and analysis of gene co-expression network in the pathogenic fungusFrontiers in microbiology · 2022Article
- BbWor1, a Regulator of Morphological Transition, Is Involved in Conidium-Hypha Switching, Blastospore Propagation, and Virulence in Beauveria bassiana.Microbiology spectrum · 2021Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biotrophic basidiomycete fungus Ustilago maydis causes smut disease in maize. Hallmarks of the disease are large tumors that develop on all aerial parts of the host in which dark pigmented teliospores are formed. We have identified a member of the WOPR family of transcription factors, Ros1, as major regulator of spore formation in U. maydis. ros1 expression is induced only late during infection and hence Ros1 is neither involved in plant colonization of dikaryotic fungal hyphae nor in plant tumor formation. However, during late stages of infection Ros1 is essential for fungal karyogamy, massive proliferation of diploid fungal cells and spore formation. Premature expression of ros1 revealed that Ros1 counteracts the b-dependent filamentation program and induces morphological alterations resembling the early steps of sporogenesis. Transcriptional profiling and ChIP-seq analyses uncovered that Ros1 remodels expression of about 30% of all U. maydis genes with 40% of these being direct targets. In total the expression of 80 transcription factor genes is controlled by Ros1. Four of the upregulated transcription factor genes were deleted and two of the mutants were affected in spore development. A large number of b-dependent genes were differentially regulated by Ros1, suggesting substantial changes in this regulatory cascade that controls filamentation and pathogenic development. Interestingly, 128 genes encoding secreted effectors involved in the establishment of biotrophic development were downregulated by Ros1 while a set of 70 "late effectors" was upregulated. These results indicate that Ros1 is a master regulator of late development in U. maydis and show that the biotrophic interaction during sporogenesis involves a drastic shift in expression of the fungal effectome including the downregulation of effectors that are essential during early stages of infection.
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