ArticlePloS one2016
Osteogenic Differentiation of Three-Dimensional Bioprinted Constructs Consisting of Human Adipose-Derived Stem Cells In Vitro and In Vivo.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 2 syntheses or guidelines pooled it, 56 citations in OpenAlex.
- Pooled it
- Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior.Frontiers in bioengineering and biotechnology · 2020Pooled it
- Corrigendum to "Strong living scaffolds for load-bearing musculoskeletal tissue regeneration" [Mater. Today Bio 35 (2025) 102571].Materials today. Bio · 2026Article
- Stem Cells in Bone Tissue Engineering: Progress, Promises and Challenges.Stem cell reviews and reports · 2024Review
- Bioprinting of gelatin-based materials for orthopedic application.Frontiers in bioengineering and biotechnology · 2024Review
- Physicochemical and Biological Characterization of Gelatin/Alginate Scaffolds Reinforced withInternational journal of biomaterials · 2024Article
- Natural Hydrogel-Based Bio-Inks for 3D Bioprinting in Tissue Engineering: A Review.Gels (Basel, Switzerland) · 2022Review
- Emerging zero-dimensional to four-dimensional biomaterials for bone regeneration.Journal of nanobiotechnology · 2022Review
- Stem Cell-Laden Hydrogel-Based 3D Bioprinting for Bone and Cartilage Tissue Engineering.Frontiers in bioengineering and biotechnology · 2022Review
- Bioprinting of Stem Cells in Multimaterial Scaffolds and Their Applications in Bone Tissue Engineering.Sensors (Basel, Switzerland) · 2021Review
- Three-Dimensional Printing of Calcium Carbonate/Hydroxyapatite Scaffolds at Low Temperature for Bone Tissue Engineering.3D printing and additive manufacturing · 2021Article
- Impact of 3D cell culture on bone regeneration potential of mesenchymal stromal cells.Stem cell research & therapy · 2021Review
- 3D Bioprinting Strategies, Challenges, and Opportunities to Model the Lung Tissue Microenvironment and Its Function.Frontiers in bioengineering and biotechnology · 2021Review
- Culture and Differentiation of Human Hair Follicle Dermal Papilla Cells in a Soft 3D Self-Assembling Peptide Scaffold.Biomolecules · 2020Article
- Comparative evaluation of the physicochemical properties of nano-hydroxyapatite/collagen and natural bone ceramic/collagen scaffolds and their osteogenesis-promoting effect on MC3T3-E1 cells.Regenerative biomaterials · 2019Article
- Suitability and limitations of mesenchymal stem cells to elucidate human bone illness.World journal of stem cells · 2019Review
- Human Adipose-Derived Hydrogel Characterization Based onStem cells international · 2019Article
- 3D bioactive composite scaffolds for bone tissue engineering.Bioactive materials · 2018Review
- 3D Bioprinting Stem Cell Derived Tissues.Cellular and molecular bioengineering · 2018Review
- 3D Printing and Electrospinning of Composite Hydrogels for Cartilage and Bone Tissue Engineering.Polymers · 2018Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we aimed to investigate osteogenic differentiation of human adipose-derived stem cells (hASCs) in three-dimensional (3D) bioprinted tissue constructs in vitro and in vivo. A 3D Bio-plotter dispensing system was used for building 3D constructs. Cell viability was determined using live/dead cell staining. After 7 and 14 days of culture, real-time quantitative polymerase chain reaction (PCR) was performed to analyze the expression of osteogenesis-related genes (RUNX2, OSX, and OCN). Western blotting for RUNX2 and immunofluorescent staining for OCN and RUNX2 were also performed. At 8 weeks after surgery, osteoids secreted by osteogenically differentiated cells were assessed by hematoxylin-eosin (H&E) staining, Masson trichrome staining, and OCN immunohistochemical staining. Results from live/dead cell staining showed that most of the cells remained alive, with a cell viability of 89%, on day 1 after printing. In vitro osteogenic induction of the 3D construct showed that the expression levels of RUNX2, OSX, and OCN were significantly increased on days 7 and 14 after printing in cells cultured in osteogenic medium (OM) compared with that in normal proliferation medium (PM). Fluorescence microscopy and western blotting showed that the expression of osteogenesis-related proteins was significantly higher in cells cultured in OM than in cells cultured in PM. In vivo studies demonstrated obvious bone matrix formation in the 3D bioprinted constructs. These results indicated that 3D bioprinted constructs consisting of hASCs had the ability to promote mineralized matrix formation and that hASCs could be used in 3D bioprinted constructs for the repair of large bone tissue defects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.