ArticleBiophysical journal2016
Micromechanical Analysis of the Hyaluronan-Rich Matrix Surrounding the Oocyte Reveals a Uniquely Soft and Elastic Composition.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- Microfluidic chips in female reproduction: a systematic review of status, advances, and challenges.Theranostics · 2024Pooled it
- Modulating IL-11-dependent matrix stiffness to delay ovarian aging.Nature aging · 2026Article
- Extracellular matrix: new insights into its role in female reproductive aging and potential therapeutic strategies.npj aging · 2026Review
- Biophysical, cellular, and mouse model approaches to investigate the mechanical regulation of folliculogenesis.Seminars in cell & developmental biology · 2025Review
- Interactions of proteins with heparan sulfate.Essays in biochemistry · 2024Review
- Cumulus expansion is impaired with advanced reproductive age due to loss of matrix integrity and reduced hyaluronan.Aging cell · 2023Article
- Deciphering Cellular Heterogeneity and Communication Patterns in Porcine Antral Follicles by Single-Cell RNA Sequencing.Animals : an open access journal from MDPI · 2023Article
- Iron overload triggering ECM-mediated Hippo/YAP pathway in follicle development: a hypothetical model endowed with therapeutic implications.Frontiers in endocrinology · 2023Review
- Surface characterization of an ultra-soft contact lens material using an atomic force microscopy nanoindentation method.Scientific reports · 2022Article
- Transcriptomic comparison of ovarian granulosa cells between adult sheep and prepubertal lambs.BMC genomics · 2022Article
- Squeezing the eggs to grow: The mechanobiology of mammalian folliculogenesis.Frontiers in cell and developmental biology · 2022Review
- Cilia take the egg on a magic carpet ride.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Scanning Probe Microscopies: Imaging and Biomechanics in Reproductive Medicine Research.International journal of molecular sciences · 2021Review
- Increasing AFM colloidal probe accuracy by optical tweezers.Scientific reports · 2021Article
- Genome-Wide Identification of mRNAs, lncRNAs, and Proteins, and Their Relationship With Sheep Fecundity.Frontiers in genetics · 2021Article
- The Inter-α-Trypsin Inhibitor Family: Versatile Molecules in Biology and Pathology.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Review
- Ovarian stiffness increases with age in the mammalian ovary and depends on collagen and hyaluronan matrices.Aging cell · 2020Article
- Ultrasound Shear Wave Velocity Varies Across Anatomical Region inTissue engineering. Part A · 2020Article
- Inter-α-inhibitor heavy chain-1 has an integrin-like 3D structure mediating immune regulatory activities and matrix stabilization during ovulation.The Journal of biological chemistry · 2020Article
- Versican G1 Fragment Establishes a Strongly Stabilized Interaction with Hyaluronan-Rich Expanding Matrix during Oocyte Maturation.International journal of molecular sciences · 2020Article
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Authors and funding
6 authors at 3 institutions in 5 countries.
Funding
Abstract
The cumulus cell-oocyte complex (COC) matrix is an extended coat that forms around the oocyte a few hours before ovulation and plays vital roles in oocyte biology. Here, we analyzed the micromechanical response of mouse COC matrix by colloidal-probe atomic force microscopy. We found that the COC matrix is elastic insofar as it does not flow and its original shape is restored after force release. At the same time, the COC matrix is extremely soft. Specifically, the most compliant parts of in vivo and in vitro expanded COC matrices yielded Young's modulus values of 0.5 ± 0.1 Pa and 1.6 ± 0.3 Pa, respectively, suggesting both high porosity and a large mesh size (≥100 nm). In addition, the elastic modulus increased progressively with indentation. Furthermore, using optical microscopy to correlate these mechanical properties with ultrastructure, we discovered that the COC is surrounded by a thick matrix shell that is essentially devoid of cumulus cells and is enhanced upon COC expansion in vivo. We propose that the pronounced nonlinear elastic behavior of the COC matrix is a consequence of structural heterogeneity and serves important functions in biological processes such as oocyte transport in the oviduct and sperm penetration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.