ArticleBiophysical journal2016
Regulating the Membrane Transport Activity and Death of Cells via Electroosmotic Manipulation.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Review
- A Versatile, Incubator-Compatible, Monolithic GaN Photonic Chipscope for Label-Free Monitoring of Live Cell Activities.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Intercellular water exchanges trigger soliton-like waves in multicellular systems.Biophysical journal · 2022Article
- Membrane Repairing Capability of Non-Small Cell Lung Cancer Cells Is Regulated by Drug Resistance and Epithelial-Mesenchymal-Transition.Membranes · 2022Article
- Active chemo-mechanical feedbacks dictate the collective migration of cells on patterned surfaces.Biophysical journal · 2022Article
- Modelling Nuclear Morphology and Shape Transformation: A Review.Membranes · 2021Review
- Mechanical properties of external confinement modulate the rounding dynamics of cells.Biophysical journal · 2021Article
- Review
- Gap junctions amplify spatial variations in cell volume in proliferating tumor spheroids.Nature communications · 2020Article
- Detection of the mesenchymal-to-epithelial transition of invasive non-small cell lung cancer cells by their membrane undulation spectra.RSC advances · 2020Article
- Nanopatterned Nafion microelectrode arrays forAdvanced functional materials · 2020Article
- Quantitative screening of the effects of hyper-osmotic stress on cancer cells cultured in 2- or 3-dimensional settings.Scientific reports · 2019Article
- An electro-osmotic microfluidic system to characterize cancer cell migration under confinement.Journal of the Royal Society, Interface · 2019Article
- Electromechanics and Volume Dynamics in Nonexcitable Tissue Cells.Biophysical journal · 2018Article
- Interrogating the Electromechanical Regulation of Cellular Volume at the Single-Cell Level.Biophysical journal · 2018Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the volume of living cells has been known to heavily influence their behavior and fate, a method allowing us to control the cell size in a programmable manner is still lacking. Here, we develop a technique in which precise changes in the cellular volume can be conveniently introduced by varying the voltage applied across a Nafion membrane that separates the culture medium from a reservoir. It is found that, unlike sudden osmotic shocks, active ion transport across the membrane of leukemia K562 cells will not be triggered by a gradual change in the extracellular osmolarity. Furthermore, when subjected to the same applied voltage, different lung and nasopharyngeal epithelial cancer cells will undergo larger volumetric changes and have a 5-10% higher death rate compared to their normal counterparts. We show that such distinct response is largely caused by the overexpression of aquaporin-4 in tumor cells, with knockout of this water channel protein resulting in a markedly reduced change in the cellular volume. Finally, by taking into account the exchange of water/ion molecules across the Nafion film and the cell membrane, a theoretical model is also proposed to describe the voltage-induced size changes of cells, which explain our experimental observations very well.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.