ArticleJournal of periodontal research2017
The epithelial cell response to health and disease associated oral biofilm models.
Article in Journal of periodontal research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 51 citations in OpenAlex.
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- Anti-Inflammatory and Osteogenic Effect of Phloroglucinol-Enriched Whey Protein Isolate Fibrillar Coating on Ti-6Al-4V Alloy.Polymers · 2025Article
- Impact of oral pathogens on inflammatory mediator expression in oral cells.Bioinformation · 2025Article
- Exploration the role of pro-inflammatory fibroblasts and related markers in periodontitis: combing with scRNA-seq and bulk-seq data.Frontiers in immunology · 2025Article
- About a Possible Impact of Endodontic Infections byInternational journal of molecular sciences · 2024Review
- Article
- Enamel Matrix Derivative Suppresses Chemokine Expression in Oral Epithelial Cells.International journal of molecular sciences · 2023Article
- Cytokines secreted by inflamed oral mucosa: implications for oral cancer progression.Oncogene · 2023Review
- Expression of Macrophage Polarization Markers against the Most Prevalent Serotypes ofMicroorganisms · 2022Article
- Role of Porphyromonas gingivalis in oral and orodigestive squamous cell carcinoma.Periodontology 2000 · 2022Review
- RNA Sequencing Reveals the Upregulation of FOXO Signaling Pathway inInternational journal of molecular sciences · 2022Article
- Comprehensive analysis of DNA methylation for periodontitis.International journal of implant dentistry · 2022Article
- Immune Tolerance in the Oral Mucosa.International journal of molecular sciences · 2021Review
- Saliva-derived microcosm biofilms grown on different oral surfaces in vitro.NPJ biofilms and microbiomes · 2021Article
- The dental plaque biofilm matrix.Periodontology 2000 · 2021Review
- Oral microbiome interactions with gingival gene expression patterns for apoptosis, autophagy and hypoxia pathways in progressing periodontitis.Immunology · 2021Article
- Comparative Analysis of Cytokine Expression in Oral Keratinocytes and THP-1 Macrophages in Response to the Most Prevalent Serotypes ofMicroorganisms · 2021Article
- Differential Response of Oral Mucosal and Gingival Cells toFrontiers in cellular and infection microbiology · 2021Article
- A review of co-culture models to study the oral microenvironment and disease.Journal of oral microbiology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectiveDifferent bacteria differentially stimulate epithelial cells. Biofilm composition and viability are likely to influence the epithelial response. In vitro model systems are commonly used to investigate periodontitis-associated bacteria and their interactions with the host; therefore, understanding factors that influence biofilm-cell interactions is essential. The present study aimed to develop in vitro monospecies and multispecies biofilms and investigate the epithelial response to these biofilms. MATERIAL AND
methodsBacterial biofilms were cultured in vitro and then either live or methanol-fixed biofilms were co-cultured with epithelial cells. Changes in epithelial cell viability, gene expression and cytokine content of culture supernatants were evaluated.
resultsBacterial viability was better preserved within mixed-species biofilm culture than within single-species biofilm culture. Both mixed- and single-species biofilms stimulated increased expression of mRNA for interleukin 8 (IL8), C-X-C motif chemokine ligand 3 (CXCL3), C-X-C motif chemokine ligand 1 (CXCL1), interleukin 1 (IL1), interleukin 6 (IL6), colony-stimulating factor 2 (CSF2) and tumour necrosis factor (TNF), and the response was greatest in response to mixed-species biofilms. Following co-culture, cytokines detected in the supernatants included IL-8, IL-6, granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor, with the greatest release of cytokines found following co-culture with methanol-fixed, mixed-species biofilms.
conclusionsThese data show that epithelial cells generate a distinct cytokine gene- and protein-expression signature in response to live or fixed, single- or multispecies biofilms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.