ArticleJournal of experimental botany2016
Light sheet microscopy reveals more gradual light attenuation in light-green versus dark-green soybean leaves.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Green light is similarly effective in promoting plant biomass as red/blue light: a meta-analysis.Journal of experimental botany · 2024Pooled it
- Stem nitrogen accumulation through vegetative storage proteins and mobilization to seeds supports high-yielding soybean.Journal of experimental botany · 2026Article
- Chemical Fingerprint ImagingAnalytical chemistry · 2025Article
- The role of red and white light in optimizing growth and accumulation of plant specialized metabolites at two light intensities in medical cannabis (Frontiers in plant science · 2024Article
- The role of chloroplast movement in C4 photosynthesis: a theoretical analysis using a three-dimensional reaction-diffusion model for maize.Journal of experimental botany · 2023Article
- A Novel Method for Estimating Chlorophyll and Carotenoid Concentrations in Leaves: A Two Hyperspectral Sensor Approach.Sensors (Basel, Switzerland) · 2023Article
- Molecular, genetic, and genomic basis of seed size and yield characteristics in soybean.Frontiers in plant science · 2023Review
- Autofluorescence-Based Investigation of Spatial Distribution of Phenolic Compounds in Soybeans Using Confocal Laser Microscopy and a High-Resolution Mass Spectrometric Approach.Molecules (Basel, Switzerland) · 2022Article
- Progress in soybean functional genomics over the past decade.Plant biotechnology journal · 2022Review
- Evidence of photosynthetic acclimation to self-shading in sugarcane canopies.Photosynthetica · 2022Article
- Light quality, oxygenic photosynthesis and more.Photosynthetica · 2022Review
- Photosynthetic acclimation during low-light-induced leaf senescence in post-anthesis maize plants.Photosynthesis research · 2021Article
- GmFULa improves soybean yield by enhancing carbon assimilation without altering flowering time or maturity.Plant cell reports · 2021Article
- Perspectives on improving light distribution and light use efficiency in crop canopies.Plant physiology · 2021Article
- A System Dynamics Approach to Model Photosynthesis at Leaf Level Under Fluctuating Light.Frontiers in plant science · 2021Article
- Subdivision of Light Signaling Networks Contributes to Partitioning of CPlant physiology · 2020Article
- Light, Not Age, Underlies the Maladaptation of Maize and Miscanthus Photosynthesis to Self-Shading.Frontiers in plant science · 2020Article
- The Spatial Distribution of Chlorophyll in Leaves.Plant physiology · 2019Article
- Physiological and transcriptomic analyses of a yellow-green mutant with high photosynthetic efficiency in wheat (Triticum aestivum L.).Functional & integrative genomics · 2018Article
- Chlorophyll Can Be Reduced in Crop Canopies with Little Penalty to Photosynthesis.Plant physiology · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light wavelengths preferentially absorbed by chlorophyll (chl) often display steep absorption gradients. This over-saturates photosynthesis in upper chloroplasts and deprives lower chloroplasts of blue and red light. Reducing chl content could create a more even leaf light distribution and thereby increase leaf light-use efficiency and overall canopy photosynthesis. This was tested on soybean cultivar 'Clark' (WT) and a near-isogenic chl b deficient mutant, Y11y11, grown in controlled environment chambers and in the field. Light attenuation was quantified using a novel approach involving light sheet microscopy. Leaf adaxial and abaxial surfaces were illuminated separately with blue, red, and green wavelengths, and chl fluorescence was detected orthogonally to the illumination plane. Relative fluorescence was significantly greater in deeper layers of the Y11y11 mesophyll than in WT, with the greatest differences in blue, then red, and finally green light when illuminated from the adaxial surface. Modeled relative photosynthesis based on chlorophyll profiles and Beer's Law predicted less steep gradients in mutant relative photosynthesis rates compared to WT. Although photosynthetic light-use efficiency was greater in the field-grown mutant with ~50% lower chl, light-use efficiency was lower in the mutant when grown in chambers where chl was ~80% reduced. This difference is probably due to pleiotropic effects of the mutation that accompany very severe reductions in chlorophyll and may warrant further testing in other low-chl lines.
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