ArticleMicrobial cell factories2016
Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 36 citations in OpenAlex.
- Metabolic Flux and Growth Profiling of Megasphaera cerevisiae for Medium-Chain Fatty Acid Synthesis.Environmental microbiology reports · 2026Article
- Defined and refined: development of a minimal medium for Clostridium pasteurianum.Applied microbiology and biotechnology · 2025Article
- A Consecutive Genome Engineering Method Reveals a New Phenotype and Regulation of Glucose and Glycerol Utilization inEngineering in life sciences · 2025Article
- Electrochemically mediated bioconversion and integrated purification greatly enhanced co-production of 1,3-propanediol and organic acids from glycerol in an industrial bioprocess.Bioprocess and biosystems engineering · 2023Article
- Phenotype analysis of cultivation processes via unsupervised machine learning: Demonstration forEngineering in life sciences · 2022Article
- Effect of Endogenous and Exogenous Butyric Acid on Butanol Production From CO by EnrichedFrontiers in bioengineering and biotechnology · 2022Article
- Disentangling the syntrophic electron transfer mechanisms of Candidatus geobacter eutrophica through electrochemical stimulation and machine learning.Scientific reports · 2021Article
- Metabolomic and kinetic investigations on the electricity-aided production of butanol byEngineering in life sciences · 2021Article
- Quantitative proteomic analysis to reveal expression differences for butanol production from glycerol and glucose by Clostridium sp. strain CT7.Microbial cell factories · 2021Article
- Increased Butanol Yields through Cosubstrate Fermentation of Jerusalem Artichoke Tubers and Crude Glycerol byACS omega · 2019Article
- Improved electrocompetence and metabolic engineering ofEngineering in life sciences · 2019Article
- A novel All-in-One electrolysis electrode and bioreactor enable better study of electrochemical effects and electricity-aided bioprocesses.Engineering in life sciences · 2018Article
- Substrates and oxygen dependent citric acid production by Yarrowia lipolytica: insights through transcriptome and fluxome analyses.Microbial cell factories · 2017Article
- Metabolic and proteomic analyses of product selectivity and redox regulation in Clostridium pasteurianum grown on glycerol under varied iron availability.Microbial cell factories · 2017Article
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Authors and funding
5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundClostridium pasteurianum is becoming increasingly attractive for the production of chemicals and fuels such as n-butanol and 1,3-propanediol. Previously we have shown that dual substrate fermentation using glucose and glycerol enhanced the cell growth and butanol production significantly. Although C. pasteurianum can grow efficiently with either glucose or glycerol alone, under certain conditions, glucose limitation in the mixed substrate fermentation leads to growth cessation. To understand this phenomenon and for process optimization, fermentation experiments were performed in the presence of excess glycerol but with varied initial concentrations of glucose which were followed by physiological, metabolic and proteomic analyses.
resultsPhysiological characterization showed that the observed cease of growth is not due to the toxicity of n-butanol. Furthermore, the growth can be resumed by addition of glucose or the intermediate oxaloacetate. Proteomic analysis shed more light on the system-level regulation of many proteins directly or indirectly associated with this phenomenon. Surprisingly, it is found that the specific growth rate of C. pasteurianum in the different growth phases (e.g. before and after glucose limitation) correlated well with the expression level of the ATP dependent pyruvate carboxylase and with the expression level of biotin synthase which provides the cofactor biotin for the formation of oxaloacetate from pyruvate. Bioenergetic analysis based on the formation rates of metabolites further show that ATP supply is not a limiting factor for the pyruvate carboxylation to oxaloacetate.
conclusionsThe results of physiological and proteomic analyses clearly show that the anaplerotic synthesis of oxaloacetate plays a key role in determining the growth behaviour of C. pasteurianum in fermentations with mixed substrates of glucose and glycerol. This study provides interesting targets for metabolic engineering of this emerging industrial microorganism.
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