ArticleScientific reports2016
Functional characterization of three trehalase genes regulating the chitin metabolism pathway in rice brown planthopper using RNA interference.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 100 citations in OpenAlex.
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- Comparative transcriptomic and metabolomics analysis of ovary in Nilaparvata lugens after trehalase inhibition.BMC genomics · 2025Article
- Comparative Transcriptomic Analysis Provides Insight into Spatiotemporal Expression Patterns of Pivotal Genes During Critical Growth Stages in Min Pig Breed.Biomolecules · 2025Article
- Chitinase inhibition induces transcriptional dysregulation altering ecdysteroid-mediated control ofiScience · 2024Article
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- Potential inhibitory effects of compounds ZK-PI-5 and ZK-PI-9 on trehalose and chitin metabolism inFrontiers in physiology · 2023Article
- Trehalose-6-phosphate synthase regulates chitin synthesis inFrontiers in physiology · 2023Article
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- Knockdown of Two Trehalase Genes by RNA Interference Is Lethal to the White-Backed PlanthopperBiomolecules · 2022Article
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7 authors at 1 institution in 1 country.
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Abstract
RNA interference (RNAi) is an effective gene-silencing tool, and double stranded RNA (dsRNA) is considered a powerful strategy for gene function studies in insects. In the present study, we aimed to investigate the function of trehalase (TRE) genes (TRE 1-1, TRE 1-2, and TRE-2) isolated from the brown planthopper Nilaparvata lugens, a typical piercing-sucking insect in rice, and investigate their regulating roles in chitin synthesis by injecting larvae with dsRNA. The results showed that TRE1 and TRE2 had compensatory function, and the expression of each increased when the other was silenced. The total rate of insects with phenotypic deformities ranged from 19.83 to 24.36% after dsTRE injection, whereas the mortality rate ranged from 14.16 to 31.78%. The mRNA levels of genes involved in the chitin metabolism pathway in RNA-Seq and DGEP, namely hexokinase (HK), glucose-6-phosphate isomerase (G6PI) and chitinase (Cht), decreased significantly at 72 h after single dsTREs injection, whereas two transcripts of chitin synthase (CHS) genes decreased at 72 h after dsTRE1-1 and dsTREs injection. These results demonstrated that TRE silencing could affect the regulation of chitin biosynthesis and degradation, causing moulting deformities. Therefore, expression inhibitors of TREs might be effective tools for the control of planthoppers in rice.
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