ArticleCellular reprogramming2016
Generation of Induced Pluripotent Stem Cells from Diabetic Foot Ulcer Fibroblasts Using a Nonintegrative Sendai Virus.
Article in Cellular reprogramming, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Stem Cells Reprogramming in Diabetes Mellitus and Diabetic Complications: Recent Advances.Current diabetes reviews · 2025Review
- Preclinical study of diabetic foot ulcers: From pathogenesis to vivo/vitro models and clinical therapeutic transformation.International wound journal · 2023Review
- Stem cell therapy in retinal diseases.Neural regeneration research · 2023Review
- Molecular Pathophysiology of Chronic Wounds: Current State and Future Directions.Cold Spring Harbor perspectives in biology · 2023Review
- [Research advances of stem cell-based tissue engineering repair materials in promoting the healing of chronic refractory wounds on the body surface].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2023Review
- Personalized Scaffolds for Diabetic Foot Ulcer Healing Using Extracellular Matrix from Induced Pluripotent Stem-Reprogrammed Patient Cells.Advanced nanobiomed research · 2022Article
- [Research progress of induced pluripotent stem cells in promoting wound healing of diabetic foot ulcers].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2022Review
- Innovative Cell and Platelet Rich Plasma Therapies for Diabetic Foot Ulcer Treatment: The Allogeneic Approach.Frontiers in bioengineering and biotechnology · 2022Review
- The Development of Tissue Engineering Scaffolds Using Matrix from iPS-Reprogrammed Fibroblasts.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Stem cell transplantation therapy for diabetic foot ulcer: a narrative review.Asian biomedicine : research, reviews and news · 2021Review
- The potential and limitations of induced pluripotent stem cells to achieve wound healing.Stem cell research & therapy · 2019Review
- Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Stem cell therapy for diabetic foot ulcers: a review of preclinical and clinical research.Stem cell research & therapy · 2018Review
- iPSC technology-based regenerative therapy for diabetes.Journal of diabetes investigation · 2018Review
- Stem Cells for Diabetes Complications: A Future Potential Cure.Rambam Maimonides medical journal · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diabetic foot ulcers (DFUs) are nonhealing chronic wounds that are a serious complication of diabetes. Since induced pluripotent stem cells (iPSCs) may offer a potent source of autologous cells to heal these wounds, we studied if repair-deficient fibroblasts, derived from DFU patients and age- and site-matched control fibroblasts, could be reprogrammed to iPSCs. To establish this, we used Sendai virus to successfully reprogram six primary fibroblast cell lines derived from ulcerated skin of two DFU patients (DFU8, DFU25), nonulcerated foot skin from two diabetic patients (DFF24, DFF9), and healthy foot skin from two nondiabetic patients (NFF12, NFF14). We confirmed reprogramming to a pluripotent state through three independent criteria: immunofluorescent staining for SSEA-4 and TRA-1-81, formation of embryoid bodies with differentiation potential to all three embryonic germ layers in vitro, and formation of teratomas in vivo. All iPSC lines showed normal karyotypes and typical, nonmethylated CpG sites for OCT4 and NANOG. iPSCs derived from DFUs were similar to those derived from site-matched nonulcerated skin from both diabetic and nondiabetic patients. These results have established for the first time that multiple, DFU-derived fibroblast cell lines can be reprogrammed with efficiencies similar to control fibroblasts, thus demonstrating their utility for future regenerative therapy of DFUs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.