Evidence map›Paper›PMID 27328202›Full record

ArticleBiomaterials science2016

Nanoparticle-mediated conversion of primary human astrocytes into neurons and oligodendrocytes.

Xiaowei Li, Kristen Kozielski, Yu-Hao Cheng, Huanhuan Liu, Camila Gadens Zamboni, Jordan Green, Hai-Quan Mao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Review
  6. Frontiers in aging neuroscience · 2021
    Review
  7. Commentary:Frontiers in cell and developmental biology · 2021
    Article
  8. Article
  9. Effects of Hydrogel-Fiber on Cystic Cavity after Spinal Cord Injury.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2019
    Article
  10. Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Xiaowei LiTranslational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA. hmao@jhu.edu and Department of Materials Science & Engineering, Johns Hopkins University, Baltimore, MD 21218, USA and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Kristen KozielskiTranslational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA. hmao@jhu.edu and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA and Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Yu-Hao ChengTranslational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA. hmao@jhu.edu and Department of Materials Science & Engineering, Johns Hopkins University, Baltimore, MD 21218, USA and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Huanhuan LiuTranslational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA. hmao@jhu.edu and Department of Materials Science & Engineering, Johns Hopkins University, Baltimore, MD 21218, USA and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Camila Gadens ZamboniTranslational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA. hmao@jhu.edu and Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Jordan GreenTranslational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA. hmao@jhu.edu and Department of Materials Science & Engineering, Johns Hopkins University, Baltimore, MD 21218, USA and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA and Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Hai-Quan MaoTranslational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA. hmao@jhu.edu and Department of Materials Science & Engineering, Johns Hopkins University, Baltimore, MD 21218, USA and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Johns Hopkins Medicine · USJohns Hopkins University · USUniversity of Baltimore · US

Funding

Wilmer Core Grant for Vision ResearchP30EY001765 · NEI · JOHNS HOPKINS UNIVERSITY · PI Ji Yi · 1985 to 2026
$22.1M
Multifunction Prosthesis Control using Implanted SensorsR01EB001672 · NIBIB · UNIVERSITY OF COLORADO DENVER · PI WEIR, RICHARD FERGUS FFRENCH · 2003 to 2015
$9.5M
Directed In Vivo Differentiation of Neural Stem Cells for Repair of Brain LesionR21NS085714 · NINDS · JOHNS HOPKINS UNIVERSITY · PI MAO, HAI-QUAN · 2014 to 2015
$446k
Nanoparticles for selective and combinatorial delivery of siRNA to brain cancerF31CA196163 · NCI · JOHNS HOPKINS UNIVERSITY · PI KOZIELSKI, KRISTEN LYNN · 2015 to 2016
$68k
NCI NIH HHS F31 CA196163NEI NIH HHS P30 EY001765NIBIB NIH HHS R01 EB001672NINDS NIH HHS R21 NS085714
6 · The paper itself

Abstract

Central nervous system (CNS) diseases and injuries are accompanied by reactive gliosis and scarring involving the activation and proliferation of astrocytes to form hypertrophic and dense structures, which present a significant barrier to neural regeneration. Engineering astrocytes to functional neurons or oligodendrocytes may constitute a novel therapeutic strategy for CNS diseases and injuries. Such direct cellular programming has been successfully demonstrated using viral vectors via the transduction of transcriptional factors, such as Sox2, which could program resident astrocytes into neurons in the adult brain and spinal cord, albeit the efficiency was low. Here we report a non-viral nanoparticle-based transfection method to deliver Sox2 or Olig2 into primary human astrocytes and demonstrate the effective conversion of the astrocytes into neurons and oligodendrocyte progenitors following the transgene expression of Sox2 and Olig2, respectively. This approach is highly translatable for engineering astrocytes to repair injured CNS tissues.

Indexed as

Cell DifferentiationAstrocytesCell EngineeringHumansNanoparticlesNeuronsOligodendrocyte Transcription Factor 2OligodendrogliaSOXB1 Transcription FactorsSpinal Cord InjuriesTransfectionOligodendrocyte Transcription Factor 2SOXB1 Transcription Factors

Identifiers

PMID27328202
PMCPMC4922536
OpenAlexW2353820411

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.