ArticleLab on a chip2016
Rapid formation of size-controllable multicellular spheroids via 3D acoustic tweezers.
Article in Lab on a chip, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
57 citing papers in PubMed, 182 citations in OpenAlex.
- Integrated microfluidic biosensors: shaping the future of quantitative life sciences and on-chip molecular diagnostics.Lab on a chip · 2026Review
- Strategies to control cellular spatial organization in microphysiological systems.Microsystems & nanoengineering · 2026Review
- Programmable acoustofluidic engineering for creating gradient biomaterials.Science advances · 2025Article
- Mass Production of Uniform Embryoid Bodies by Acoustic Standing Waves.Small methods · 2025Article
- 3D bioprinting in tissue engineering: current state-of-the-art and challenges towards system standardization and clinical translation.Biofabrication · 2025Review
- Organoid-based tissue engineering for advanced tissue repair and reconstruction.Materials today. Bio · 2025Review
- Real-time color flow mapping of ultrasound microrobots.Science advances · 2025Article
- An acoustic levitation platform for high-content histological analysis of 3D tissue culture.Lab on a chip · 2025Article
- Emerging biotechnologies for engineering liver organoids.Bioactive materials · 2025Review
- Sound innovations for biofabrication and tissue engineering.Microsystems & nanoengineering · 2024Review
- Simple and Rapid Method of Microwell Array Fabrication for Drug Testing on 3D Cancer Spheroids.ACS omega · 2024Article
- Acoustofluidic Actuation of Living Cells.Micromachines · 2024Review
- Ultrasound-induced reorientation for multi-angle optical coherence tomography.Nature communications · 2024Article
- Exploiting Sound for Emerging Applications of Extracellular Vesicles.Nano research · 2024Article
- Engineering Heterogeneous Tumor Models for Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- In vivo acoustic patterning of endothelial cells for tissue vascularization.Scientific reports · 2023Article
- Acoustofluidic assembly of primary tumor-derived organotypic cell clusters for rapid evaluation of cancer immunotherapy.Journal of nanobiotechnology · 2023Article
- Fabrication Scaffold with High Dimensional Control for Spheroids with Undifferentiated iPS Cell Properties.Cells · 2023Article
- High-throughput fabrication of cell spheroids with 3D acoustic assembly devices.International journal of bioprinting · 2023Article
- Noninvasive prenatal diagnosis targeting fetal nucleated red blood cells.Journal of nanobiotechnology · 2022Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
The multicellular spheroid is an important 3D cell culture model for drug screening, tissue engineering, and fundamental biological research. Although several spheroid formation methods have been reported, the field still lacks high-throughput and simple fabrication methods to accelerate its adoption in drug development industry. Surface acoustic wave (SAW) based cell manipulation methods, which are known to be non-invasive, flexible, and high-throughput, have not been successfully developed for fabricating 3D cell assemblies or spheroids, due to the limited understanding on SAW-based vertical levitation. In this work, we demonstrated the capability of fabricating multicellular spheroids in the 3D acoustic tweezers platform. Our method used drag force from microstreaming to levitate cells in the vertical direction, and used radiation force from Gor'kov potential to aggregate cells in the horizontal plane. After optimizing the device geometry and input power, we demonstrated the rapid and high-throughput nature of our method by continuously fabricating more than 150 size-controllable spheroids and transferring them to Petri dishes every 30 minutes. The spheroids fabricated by our 3D acoustic tweezers can be cultured for a week with good cell viability. We further demonstrated that spheroids fabricated by this method could be used for drug testing. Unlike the 2D monolayer model, HepG2 spheroids fabricated by the 3D acoustic tweezers manifested distinct drug resistance, which matched existing reports. The 3D acoustic tweezers based method can serve as a novel bio-manufacturing tool to fabricate complex 3D cell assembles for biological research, tissue engineering, and drug development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.