ArticleACS chemical biology2016
Dark Hydrazone Fluorescence Labeling Agents Enable Imaging of Cellular Aldehydic Load.
Article in ACS chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Evolutionary Specialization of a Promiscuous Designer Enzyme.ACS catalysis · 2025Article
- Tunable fluorescent probes for detecting aldehydes in living systems.Chemical science · 2024Article
- Chemical sensors for imaging total cellular aliphatic aldehydes in live cells.Chemical science · 2023Article
- Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity.Chemical reviews · 2023Review
- The Need to Pair Molecular Monitoring Devices with Molecular Imaging to Personalize Health.Molecular imaging and biology · 2022Review
- Microbial byproducts determine reproductive fitness of free-living and parasitic nematodes.Cell host & microbe · 2022Article
- Discovery of Potent and Selective Inhibitors against Protein-Derived Electrophilic Cofactors.Journal of the American Chemical Society · 2022Article
- Rapid glycoconjugation with glycosyl amines.Chemical science · 2021Article
- One-pot oxime ligation from peptides bearing thiazolidine and aminooxyacetyl groups.RSC advances · 2020Article
- A fluorescent hydrazone exchange probe of pyridoxal phosphate for the assessment of vitamin B6 status.Chemical communications (Cambridge, England) · 2019Article
- Bioanalytical and Mass Spectrometric Methods for Aldehyde Profiling in Biological Fluids.Toxics · 2019Review
- Redox Signaling by Reactive Electrophiles and Oxidants.Chemical reviews · 2018Review
- Exceptionally rapid oxime and hydrazone formation promoted by catalytic amine buffers with low toxicity.Chemical science · 2018Article
- Aldehyde dehydrogenase 3A1 activation prevents radiation-induced xerostomia by protecting salivary stem cells from toxic aldehydes.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Aldehyde-Induced DNA and Protein Adducts as Biomarker Tools for Alcohol Use Disorder.Trends in molecular medicine · 2018Review
- Discovery of a novel calcium-sensitive fluorescent probe for α-ketoglutarate.Acta pharmacologica Sinica · 2017Article
- Oximes and Hydrazones in Bioconjugation: Mechanism and Catalysis.Chemical reviews · 2017Review
- Detecting Protein ADP-Ribosylation Using a Clickable Aminooxy Probe.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Aldehyde dehydrogenase 2 in aplastic anemia, Fanconi anemia and hematopoietic stem cells.Molecular genetics and metabolism · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Aldehydes are key intermediates in many cellular processes, from endogenous metabolic pathways like glycolysis to undesired exogenously induced processes such as lipid peroxidation and DNA interstrand cross-linking. Alkyl aldehydes are well documented to be cytotoxic, affecting the functions of DNA and protein, and their levels are tightly regulated by the oxidative enzyme ALDH2. Mutations in this enzyme are associated with cardiac damage, diseases such as Fanconi anemia (FA), and cancer. Many attempts have been made to identify and quantify the overall level of these alkyl aldehydes inside cells, yet there are few practical methods available to detect and monitor these volatile aldehydes in real time. Here, we describe a multicolor fluorogenic hydrazone transfer ("DarkZone") system to label alkyl aldehydes, yielding up to 30-fold light-up response in vitro. A cell-permeant DarkZone dye design was applied to detect small-molecule aldehydes in the cellular environment. The new dye design also enabled the monitoring of cellular acetaldehyde production from ethanol over time by flow cytometry, demonstrating the utility of the DarkZone dyes for measuring and imaging the aldehydic load related to human disease.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.