Evidence map›Paper›PMID 27325916›Full record

ArticleGut pathogens2016

Complete genome sequence of Vibrio vulnificus FORC_017 isolated from a patient with a hemorrhagic rash after consuming raw dotted gizzard shad.

Han Young Chung, You-Tae Kim, Suyeon Kim, Eun Jung Na, Hye-Jin Ku, Keun Hwa Lee, Sang Taek Heo, Sangryeol Ryu, Heebal Kim, Sang Ho Choi and 1 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Gut pathogens, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Han Young ChungDepartment of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul, Republic of Korea.
You-Tae KimDepartment of Food Science and Biotechnology, Kyung Hee University, Yongin, Republic of Korea.
Suyeon KimDepartment of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul, Republic of Korea.
Eun Jung NaDepartment of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul, Republic of Korea.
Hye-Jin KuDepartment of Food Science and Biotechnology, Kyung Hee University, Yongin, Republic of Korea.
Keun Hwa LeeDepartment of Internal Medicine, Jeju National University School of Medicine, Jeju, Republic of Korea.
Sang Taek HeoDepartment of Internal Medicine, Jeju National University School of Medicine, Jeju, Republic of Korea.
Sangryeol RyuDepartment of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul, Republic of Korea.
Heebal KimDepartment of Animal Science and Biotechnology, Seoul National University, Seoul, Republic of Korea.
Sang Ho ChoiDepartment of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul, Republic of Korea.
Ju-Hoon LeeDepartment of Food Science and Biotechnology, Kyung Hee University, Yongin, Republic of Korea.
Seoul National University · KRKyung Hee University · KRJeju National University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundVibrio vulnificus, a resident in the human gut, is frequently found in seafood, causing food-borne illnesses including gastroenteritis and severe septicemia. While V. vulnificus has been known to be one of the major food-borne pathogens, pathogenicity and virulence factors are not fully understood yet. To extend our understanding of the pathogenesis of V. vulnificus at the genomic level, the genome of V. vulnificus FORC_017 isolated from a female patient experiencing a hemorrhagic rash was completely sequenced and analyzed.

resultsThree discontinuous contigs were generated from a hybrid assembly using Illumina MiSeq and PacBio platforms, revealing that the genome of the FORC_017 consists of two circular chromosomes and a plasmid. Chromosome I consists of 3,253,417-bp (GC content 46.49 %) containing 2943 predicted open reading frames (ORFs) and chromosome II of 1,905,745-bp (GC content 46.90 %) containing 1638 ORFs. The plasmid pFORC17 consists of 70,069-bp (GC content 43.77 %) containing 84 ORFs. The average nucleotide identity (ANI) value of the FORC_017 and CMCP6 strains was 98.53, suggesting that they are closely related.

conclusionsPathogenesis-associated genes including vvhA, rtx gene cluster, and various hemolysin genes were present in FORC_017. In addition, three complete secretion systems (Type I, II and VI) as well as iron uptake-related genes for virulence of the FORC_017 were detected, suggesting that this strain is pathogenic. Further comparative genome analysis revealed that FORC_017 and CMCP6 share major toxin genes including vvhA and rtx for pathogenesis activities. The genome information of the FORC_017 provides novel insights into pathogenicity and virulence factors of V. vulnificus.

Indexed as

Genome sequencingHemolysinHybrid assemblyPathogenesisSecretion systemVibrio vulnificusVirulence factor

Identifiers

PMID27325916
PMCPMC4913425
OpenAlexW2396397096

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.