Evidence map›Paper›PMID 27325759›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2016

Distinct oligoclonal band antibodies in multiple sclerosis recognize ubiquitous self-proteins.

Simone M Brändle, Birgit Obermeier, Makbule Senel, Jessica Bruder, Reinhard Mentele, Mohsen Khademi, Tomas Olsson, Hayrettin Tumani, Wolfgang Kristoferitsch, Friedrich Lottspeich and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
103citing papers in PubMed, 2 pooled it
16.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

103 citing papers in PubMed, 2 syntheses or guidelines pooled it, 190 citations in OpenAlex.

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  15. Modulator of VRAC Current 1 Is a Potential Target Antigen in Multiple Sclerosis.Neurology(R) neuroimmunology & neuroinflammation · 2025
    Article
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43 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 6 institutions in 3 countries.

Simone M BrändleInstitute of Clinical Neuroimmunology, Ludwig-Maximilians-University, 81377 Munich, Germany;
Birgit ObermeierInstitute of Clinical Neuroimmunology, Ludwig-Maximilians-University, 81377 Munich, Germany;
Makbule SenelInstitute of Clinical Neuroimmunology, Ludwig-Maximilians-University, 81377 Munich, Germany; Department of Neurology, University of Ulm, 89081 Ulm, Germany;
Jessica BruderInstitute of Clinical Neuroimmunology, Ludwig-Maximilians-University, 81377 Munich, Germany;
Reinhard MenteleInstitute of Clinical Neuroimmunology, Ludwig-Maximilians-University, 81377 Munich, Germany;
Mohsen KhademiNeuroimmunology Unit, Department of Clinical Neurosciences, Center for Molecular Medicine CMM, Karolinska University Hospital, 171 76 Stockholm, Sweden;
Tomas OlssonNeuroimmunology Unit, Department of Clinical Neurosciences, Center for Molecular Medicine CMM, Karolinska University Hospital, 171 76 Stockholm, Sweden;
Hayrettin TumaniDepartment of Neurology, University of Ulm, 89081 Ulm, Germany;
Wolfgang KristoferitschKarl Landsteiner Institute for Neuroimmunological and Neurodegenerative Disorders, Sozialmedizinisches Zentrum Donauspital, 1220 Vienna, Austria;
Friedrich LottspeichDepartment for Protein Analytics, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany;
Hartmut WekerleHertie Senior Professor Group Neuroimmunology, Max-Planck-Institute of Neurobiology, 82152 Martinsried, Germany; Munich Cluster for Systems Neurology (SyNergy), Ludwig-Maximilians-University, 81377 Munich, Germany.
Reinhard HohlfeldInstitute of Clinical Neuroimmunology, Ludwig-Maximilians-University, 81377 Munich, Germany; Munich Cluster for Systems Neurology (SyNergy), Ludwig-Maximilians-University, 81377 Munich, Germany.
Klaus DornmairInstitute of Clinical Neuroimmunology, Ludwig-Maximilians-University, 81377 Munich, Germany; Munich Cluster for Systems Neurology (SyNergy), Ludwig-Maximilians-University, 81377 Munich, Germany klaus.dornmair@med.uni-muenchen.de.
Ludwig-Maximilians-Universität München · DEKarolinska University Hospital · SEMunich Cluster for Systems Neurology · DEDonauspital · ATMax Planck Institute of Biochemistry · DEUniversität Ulm · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oligoclonal Ig bands (OCBs) of the cerebrospinal fluid are a hallmark of multiple sclerosis (MS), a disabling inflammatory disease of the central nervous system (CNS). OCBs are locally produced by clonally expanded antigen-experienced B cells and therefore are believed to hold an important clue to the pathogenesis. However, their target antigens have remained unknown, mainly because it was thus far not possible to isolate distinct OCBs against a background of polyclonal antibodies. To overcome this obstacle, we copurified disulfide-linked Ig heavy and light chains from distinct OCBs for concurrent analysis by mass spectrometry and aligned patient-specific peptides to corresponding transcriptome databases. This method revealed the full-length sequences of matching chains from distinct OCBs, allowing for antigen searches using recombinant OCB antibodies. As validation, we demonstrate that an OCB antibody from a patient with an infectious CNS disorder, neuroborreliosis, recognized a Borrelia protein. Next, we produced six recombinant antibodies from four MS patients and identified three different autoantigens. All of them are conformational epitopes of ubiquitous intracellular proteins not specific to brain tissue. Our findings indicate that the B-cell response in MS is heterogeneous and partly directed against intracellular autoantigens released during tissue destruction. In addition to helping elucidate the role of B cells in MS, our approach allows the identification of target antigens of OCB antibodies in other neuroinflammatory diseases and the production of therapeutic antibodies in infectious CNS diseases.

Indexed as

AutoantigensBorreliaHEK293 CellsHumansLyme NeuroborreliosisMultiple SclerosisOligoclonal BandsAutoantigensOligoclonal Bandsautoantigensmultiple sclerosisoligoclonal bandsproteomicstranscriptomics

Identifiers

PMID27325759
PMCPMC4948369
OpenAlexW2461595816

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.