ArticlePlant physiology2016
The Relationship of Triacylglycerol and Starch Accumulation to Carbon and Energy Flows during Nutrient Deprivation in Chlamydomonas reinhardtii.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 54 citations in OpenAlex.
- Nominal Concentration-Based Assessment of Structure-Dependent PFAS Responses in the Freshwater DiatomJournal of xenobiotics · 2026Article
- Gelatinization and Pasting Property of Small Granular Starch fromGels (Basel, Switzerland) · 2026Article
- Energy metabolism and adaptation to hypoxia in the non-photosynthetic green alga Leontynka.BMC biology · 2026Article
- A multi-omic characterization of the physiological responses to salt stress in Scenedesmus obliquus UTEX393.Communications biology · 2025Article
- Transcriptomic and Metabolic Analysis Reveal Potential Mechanism of Starch Accumulation inPlants (Basel, Switzerland) · 2025Article
- Artificial Intelligence in Advancing Algal Bioactive Ingredients: Production, Characterization, and Application.Foods (Basel, Switzerland) · 2025Review
- Harnessing genetic engineering to drive economic bioproduct production in algae.Frontiers in bioengineering and biotechnology · 2024Review
- Cultivation of Pseudochlorella pringsheimii for biodiesel production in a scalable indoor photobioreactor: case studies from Egypt.Journal, genetic engineering & biotechnology · 2023Article
- The lifetime of the oxygen-evolving complex subunit PSBO depends on light intensity and carbon availability in Chlamydomonas.Plant, cell & environment · 2023Article
- 13C-labeling reveals how membrane lipid components contribute to triacylglycerol accumulation in Chlamydomonas.Plant physiology · 2022Article
- Differences in Glycerolipid Response ofFrontiers in plant science · 2022Article
- Article
- Large fluxes of fatty acids from membranes to triacylglycerol and back during N-deprivation and recovery in Chlamydomonas.Plant physiology · 2021Article
- Dynamism of Metabolic Carbon Flow of Starch and Lipids inFrontiers in plant science · 2021Article
- Responses of a Newly Evolved Auxotroph of Chlamydomonas to BPlant physiology · 2020Article
- Symbiont population control by host-symbiont metabolic interaction in Symbiodiniaceae-cnidarian associations.Nature communications · 2020Article
- Optimization of a simple, accurate and low cost method for starch quantification in green microalgae.Botanical studies · 2019Article
- Subcellular Energetics and Carbon Storage inCells · 2019Review
- Impairment of starch biosynthesis results in elevated oxidative stress and autophagy activity in Chlamydomonas reinhardtii.Scientific reports · 2019Article
- Nitrogen-dependent coordination of cell cycle, quiescence and TAG accumulation in Chlamydomonas.Biotechnology for biofuels · 2019Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Because of the potential importance of algae for green biotechnology, considerable effort has been invested in understanding their responses to nitrogen deprivation. The most frequently invoked reasons proposed for the accumulation of high cellular levels of triacylglycerol (TAG) and starch are variants of what may be termed the "overflow hypothesis." According to this, growth inhibition results in the rate of photosynthetic energy and/or carbon input exceeding cellular needs; the excess input is directed into the accumulation of TAG and/or starch to prevent damage. This study was aimed at providing a quantitative dataset and analysis of the main energy and carbon flows before and during nitrogen deprivation in a model system to assess alternative explanations. Cellular growth, biomass, starch, and lipid levels as well as several measures of photosynthetic function were recorded for cells of Chlamydomonas reinhardtii cultured under nine different autotrophic, mixotrophic, and heterotrophic conditions during nutrient-replete growth and for the first 4 d of nitrogen deprivation. The results of a (13)C labeling time course indicated that in mixotrophic culture, starch is predominantly made from CO2 and fatty acid synthesis is largely supplied by exogenous acetate, with considerable turnover of membrane lipids, so that total lipid rather than TAG is the appropriate measure of product accumulation. Heterotrophic cultures accumulated TAG and starch during N deprivation, showing that these are not dependent on photosynthesis. We conclude that the overflow hypothesis is insufficient and suggest that storage may be a more universally important reason for carbon compound accumulation during nutrient deprivation.
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