Evidence map›Paper›PMID 27325438›Full record

ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2016

Whole genome sequencing as a tool for phylogenetic analysis of clinical strains of Mitis group streptococci.

L H Rasmussen, R Dargis, K Højholt, J J Christensen, O Skovgaard, U S Justesen, F S Rosenvinge, C Moser, O Lukjancenko, S Rasmussen and 1 more

Abstract read
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In one paragraph

Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.2field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 25 citations in OpenAlex.

  1. Re-evaluation of boundaries ofInternational journal of systematic and evolutionary microbiology · 2025
    Article
  2. Article
  3. Microbiology spectrum · 2024
    Article
  4. Insights into the role ofFrontiers in cellular and infection microbiology · 2024
    Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Identification of Streptococcus pneumoniae and other Mitis streptococci: importance of molecular methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020
    Review
  12. Relationships among streptococci from the mitis group, misidentified as Streptococcus pneumoniae.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020
    Article
  13. Article
  14. Article
  15. Article
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

L H RasmussenDepartment of Clinical Microbiology, Slagelse Hospital, Slagelse, Denmark.
R DargisDepartment of Clinical Microbiology, Slagelse Hospital, Slagelse, Denmark.
K HøjholtDepartment of Clinical Microbiology, Slagelse Hospital, Slagelse, Denmark.
J J ChristensenDepartment of Clinical Microbiology, Slagelse Hospital, Slagelse, Denmark.
O SkovgaardDepartment of Science and Environment, Roskilde University, Roskilde, Denmark.
U S JustesenDepartment of Clinical Microbiology, Odense University Hospital, Odense, Denmark.
F S RosenvingeDepartment of Clinical Microbiology, Vejle Hospital, Vejle, Denmark.
C MoserDepartment of Clinical Microbiology, University Hospital of Copenhagen, Copenhagen, Denmark.
O LukjancenkoNational Food Institute, Technical University of Denmark, Copenhagen, Denmark.
S RasmussenDTU Systems Biology, Technical University of Denmark, Copenhagen, Denmark.
X C NielsenDepartment of Clinical Microbiology, Slagelse Hospital, Slagelse, Denmark. xcn@regionsjaelland.dk.
Slagelse Hospital · DKTechnical University of Denmark · DKCopenhagen University Hospital · DKRoskilde University · DKVejle Sygehus · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Identification of Mitis group streptococci (MGS) to the species level is challenging for routine microbiology laboratories. Correct identification is crucial for the diagnosis of infective endocarditis, identification of treatment failure, and/or infection relapse. Eighty MGS from Danish patients with infective endocarditis were whole genome sequenced. We compared the phylogenetic analyses based on single genes (recA, sodA, gdh), multigene (MLSA), SNPs, and core-genome sequences. The six phylogenetic analyses generally showed a similar pattern of six monophyletic clusters, though a few differences were observed in single gene analyses. Species identification based on single gene analysis showed their limitations when more strains were included. In contrast, analyses incorporating more sequence data, like MLSA, SNPs and core-genome analyses, provided more distinct clustering. The core-genome tree showed the most distinct clustering.

Indexed as

Genetic VariationGenome, BacterialPhylogenySequence Analysis, DNACluster AnalysisDenmarkEndocarditisHumansRetrospective StudiesStreptococcal InfectionsStreptococcus mitis

Identifiers

PMID27325438
OpenAlexW2466459805

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.