ArticleScientific reports2016
Efficient generation of transgenic cattle using the DNA transposon and their analysis by next-generation sequencing.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
- Highly efficient and low-mosaicismiScience · 2026Article
- Electroporation-mediated site-specific knock-in of a human coding sequence into the bovine ALB locus in embryos.BMC biotechnology · 2026Article
- MSTN knockout enhances the production of MYOD1-mediated steak-type cultivated meat.Journal of animal science and biotechnology · 2025Article
- Cas9-expressing cattle using the PiggyBac transposon all-in-one system.BMC genomics · 2025Article
- Mammalian ubiquitous promoter isolated from proximal regulatory region of bovine MSTN gene.Scientific reports · 2024Article
- Long-term (10-year) monitoring of transposon-mediated transgenic cattle.Transgenic research · 2024Article
- Stable long-term germline transmission of GFP transgenic rat via PiggyBac transposon mediated gene transfer.BMC veterinary research · 2024Article
- Outlook on genome editing application to cattle.Journal of veterinary science · 2024Review
- Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein (RNP) electroporation.Journal of animal science and biotechnology · 2023Article
- Hereditary Basis of Coat Color and Excellent Feed Conversion Rate of Red Angus Cattle by Next-Generation Sequencing Data.Animals : an open access journal from MDPI · 2022Article
- Application of transposon systems in the transgenesis of bovine somatic and germ cells.BMC veterinary research · 2022Article
- Cows as Bioreactors for the Production of Nutritionally and Biomedically Significant Proteins.Advances in experimental medicine and biology · 2022Article
- Using Genome Scale Mutant Libraries to Identify Essential Genes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- CRISPR/Cas9-mediated knock-in strategy at the Rosa26 locus in cattle fetal fibroblasts.PloS one · 2022Article
- Contemporary Transposon Tools: A Review and Guide through Mechanisms and Applications ofInternational journal of molecular sciences · 2021Review
- In VivoInternational journal of molecular sciences · 2019Article
- Production of hypoallergenic milk from DNA-free beta-lactoglobulin (BLG) gene knockout cow using zinc-finger nucleases mRNA.Scientific reports · 2018Article
- Efficient targeted integration into the bovine Rosa26 locus using TALENs.Scientific reports · 2018Article
- Identification of transposable elements fused in the exonic region of the olive flounder genome.Genes & genomics · 2018Article
- Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer.BMC genomics · 2018Article
Corrections and comments
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Authors and funding
18 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we efficiently generated transgenic cattle using two transposon systems (Sleeping Beauty and Piggybac) and their genomes were analyzed by next-generation sequencing (NGS). Blastocysts derived from microinjection of DNA transposons were selected and transferred into recipient cows. Nine transgenic cattle have been generated and grown-up to date without any health issues except two. Some of them expressed strong fluorescence and the transgene in the oocytes from a superovulating one were detected by PCR and sequencing. To investigate genomic variants by the transgene transposition, whole genomic DNA were analyzed by NGS. We found that preferred transposable integration (TA or TTAA) was identified in their genome. Even though multi-copies (i.e. fifteen) were confirmed, there was no significant difference in genome instabilities. In conclusion, we demonstrated that transgenic cattle using the DNA transposon system could be efficiently generated, and all those animals could be a valuable resource for agriculture and veterinary science.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.