ArticleNature cell biology2016
Staccato/Unc-13-4 controls secretory lysosome-mediated lumen fusion during epithelial tube anastomosis.
Article in Nature cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- The endo-lysosomal system drives lumen formation in a human epiblast model.Nature communications · 2026Article
- Distinct Rab11-associated membrane trafficking pathways bidirectionally control neuronal extracellular vesicle cargo traffic.bioRxiv : the preprint server for biology · 2026Article
- The dynamics of tubulogenesis in development and disease.Development (Cambridge, England) · 2025Review
- Cell-Mediated Branch Fusion in the Drosophila Trachea.Results and problems in cell differentiation · 2024Review
- A Rab39-Klp98A-Rab35 endocytic recycling pathway is essential for rapid Golgi-dependent furrow ingression.Development (Cambridge, England) · 2023Article
- The Osiris family genes function as novel regulators of the tube maturation process in the Drosophila trachea.PLoS genetics · 2023Article
- Morphogenetic Roles of Hydrostatic Pressure in Animal Development.Annual review of cell and developmental biology · 2022Review
- Integrin α3β1 promotes vessel formation of glioblastoma-associated endothelial cells through calcium-mediated macropinocytosis and lysosomal exocytosis.Nature communications · 2022Article
- Crosstalk between basal extracellular matrix adhesion and building of apical architecture during morphogenesis.Biology open · 2021Review
- Drosophila Rab39 Attenuates Lysosomal Degradation.International journal of molecular sciences · 2021Article
- Transcytosis via the late endocytic pathway as a cell morphogenetic mechanism.The EMBO journal · 2020Article
- Article
- Multiple Requirements for Rab GTPases in the Development ofG3 (Bethesda, Md.) · 2020Article
- Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry intoeLife · 2019Article
- Rabs on the fly: Functions of Rab GTPases during development.Small GTPases · 2019Review
- Rab-mediated trafficking in the secondary cells of Drosophila male accessory glands and its role in fecundity.Traffic (Copenhagen, Denmark) · 2019Article
- Development and Function of theGenetics · 2018Review
- Insane in the apical membrane: Trafficking events mediating apicobasal epithelial polarity during tube morphogenesis.Traffic (Copenhagen, Denmark) · 2018Review
- SERCA directs cell migration and branching across species and germ layers.Biology open · 2017Article
- Multiphoton in vivo imaging with a femtosecond semiconductor disk laser.Biomedical optics express · 2017Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A crucial yet ill-defined step during the development of tubular networks, such as the vasculature, is the formation of connections (anastomoses) between pre-existing lumenized tubes. By studying tracheal tube anastomosis in Drosophila melanogaster, we uncovered a key role of secretory lysosome-related organelle (LRO) trafficking in lumen fusion. We identified the conserved calcium-binding protein Unc-13-4/Staccato (Stac) and the GTPase Rab39 as critical regulators of this process. Stac and Rab39 accumulate on dynamic vesicles, which form exclusively in fusion tip cells, move in a dynein-dependent manner, and contain late-endosomal, lysosomal, and SNARE components characteristic of LROs. The GTPase Arl3 is necessary and sufficient for Stac LRO formation and promotes Stac-dependent intracellular fusion of juxtaposed apical plasma membranes, thereby forming a transcellular lumen. Concomitantly, calcium is released locally from ER exit sites and apical membrane-associated calcium increases. We propose that calcium-dependent focused activation of LRO exocytosis restricts lumen fusion to appropriate domains within tip cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.