ArticleScientific reports2016
Selection of reliable reference genes for RT-qPCR analysis during developmental stages and abiotic stress in Setaria viridis.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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57 citing papers in PubMed.
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- Selection and validation of reference genes for qRT-PCR normalization in dayflower (Commelina communis) based on the transcriptome profiling.BMC plant biology · 2024Article
- Understanding the responses of tillering to 2,4-D isooctyl ester in Setaria viridis L.BMC genomics · 2024Article
- How ambient temperature affects the heading date of foxtail millet (Frontiers in plant science · 2023Article
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- The Characteristics of Mercury Flux at the Interfaces between Two Typical Plants and the Air inInternational journal of environmental research and public health · 2021Article
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9 authors.
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Abstract
Real-time PCR (RT-qPCR) expression analysis is a powerful analytical technique, but reliable results depend on the use of stable reference genes for proper normalization. This study proposed to test the expression stability of 13 candidate reference genes in Setaria viridis, a monocot species recently proposed as a new C4 model plant. Gene expression stability of these genes was assayed across different tissues and developmental stages of Setaria and under drought or aluminum stress. In general, our results showed Protein Kinase, RNA Binding Protein and SDH as the most stable genes. Moreover, pairwise analysis showed that two reference genes were sufficient to normalize the gene expression data under each condition. By contrast, GAPDH and ACT were the least stably expressed genes tested. Validation of suitable reference genes was carried out to profile the expression of P5CS and GolS during abiotic stress. In addition, normalization of gene expression of SuSy, involved in sugar metabolism, was assayed in the developmental dataset. This study provides a list of reliable reference genes for transcript normalization in S. viridis in different tissues and stages of development and under abiotic stresses, which will facilitate genetic studies in this monocot model plant.
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